Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW46_RS01285 Genome accession   NZ_CP036085
Coordinates   231845..235570 (-) Length   1241 a.a.
NCBI ID   WP_215576874.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS12/5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 226845..240570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW46_RS01240 (EXW46_01255) - 228303..228482 (+) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -
  EXW46_RS01245 (EXW46_01260) gerPA 228579..228800 (+) 222 WP_001111190.1 spore germination protein GerPA -
  EXW46_RS01250 (EXW46_01265) gerPB 228815..229021 (+) 207 WP_044437744.1 spore germination protein GerPB -
  EXW46_RS01255 (EXW46_01270) - 229089..229703 (+) 615 WP_215576875.1 spore germination protein GerPC -
  EXW46_RS01260 (EXW46_01275) - 229710..229904 (+) 195 WP_113734011.1 spore gernimation protein GerPD -
  EXW46_RS01265 (EXW46_01280) - 229919..230305 (+) 387 WP_113734010.1 spore germination protein GerPE -
  EXW46_RS01270 (EXW46_01285) gerPF 230348..230563 (+) 216 WP_001141566.1 spore germination protein GerPF -
  EXW46_RS01275 (EXW46_01290) - 230620..231327 (-) 708 WP_113734009.1 DNA alkylation repair protein -
  EXW46_RS01280 (EXW46_01295) - 231543..231821 (-) 279 WP_113734008.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW46_RS01285 (EXW46_01300) addA 231845..235570 (-) 3726 WP_215576874.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW46_RS01290 (EXW46_01305) addB 235567..239082 (-) 3516 WP_016093092.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW46_RS01295 (EXW46_01310) lepB 239200..239763 (-) 564 WP_016119804.1 signal peptidase I -
  EXW46_RS01300 (EXW46_01315) - 239974..240567 (-) 594 WP_002140801.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142539.85 Da        Isoelectric Point: 4.9179

>NTDB_id=346565 EXW46_RS01285 WP_215576874.1 231845..235570(-) (addA) [Bacillus mycoides strain JAS12/5]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEGGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYAVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYSASWK
VEVVEGKDLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYADATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIDE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=346565 EXW46_RS01285 WP_215576874.1 231845..235570(-) (addA) [Bacillus mycoides strain JAS12/5]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACAATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAACTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCGCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACTTTAA
AGCGTATTAAAAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGGCGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCGATTTACCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGACGGCGGAATGAAAATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCTGCTGAACTATTATGCA
TTCAGCAAACTGAGGAAGAAGTGCAAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGCTATGCAGTGTATGATCGTAAAACTGATAGTATGCGCCCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTCATTGATAAC
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAGGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGATCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTGCGCTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCCCTCGGAGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATTCGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGGCGAAATTTATGAATATAGTGCTAGCTGGAAA
GTAGAAGTTGTTGAAGGAAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
CGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGGAATACGGGTATGCGG
ACGCGACATCTCACCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGAAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.537

Multiple sequence alignment