Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW45_RS22115 Genome accession   NZ_CP036073
Coordinates   4320163..4321206 (-) Length   347 a.a.
NCBI ID   WP_060488444.1    Uniprot ID   A0A2B5SJ93
Organism   Bacillus wiedmannii strain JAS08/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4315163..4326206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW45_RS22070 (EXW45_22470) - 4315593..4315793 (-) 201 WP_000106080.1 YqzE family protein -
  EXW45_RS22075 (EXW45_22475) - 4315832..4316329 (-) 498 WP_098208622.1 shikimate kinase -
  EXW45_RS22080 (EXW45_22480) - 4316448..4317098 (-) 651 WP_046957539.1 2OG-Fe(II) oxygenase -
  EXW45_RS22085 (EXW45_22485) comGG 4317273..4317644 (-) 372 WP_088123395.1 competence type IV pilus minor pilin ComGG -
  EXW45_RS22090 (EXW45_22490) comGF 4317641..4318111 (-) 471 WP_000923108.1 competence type IV pilus minor pilin ComGF -
  EXW45_RS22095 (EXW45_22495) - 4318081..4318383 (-) 303 WP_097915827.1 competence protein ComG -
  EXW45_RS22100 (EXW45_22500) comGD 4318376..4318831 (-) 456 WP_000810391.1 comG operon protein ComGD -
  EXW45_RS22105 (EXW45_22505) comGC 4318828..4319127 (-) 300 WP_001178693.1 competence type IV pilus major pilin ComGC -
  EXW45_RS22110 (EXW45_22510) comGB 4319139..4320170 (-) 1032 WP_098085116.1 competence type IV pilus assembly protein ComGB -
  EXW45_RS22115 (EXW45_22515) comGA 4320163..4321206 (-) 1044 WP_060488444.1 competence protein ComGA Machinery gene
  EXW45_RS22120 (EXW45_22520) - 4321413..4322108 (+) 696 WP_000434130.1 helix-turn-helix domain-containing protein -
  EXW45_RS22125 (EXW45_22525) - 4322233..4322475 (+) 243 WP_000440719.1 DUF2626 domain-containing protein -
  EXW45_RS22130 (EXW45_22530) - 4322582..4323976 (+) 1395 WP_046956341.1 L-cystine transporter -
  EXW45_RS22135 (EXW45_22535) - 4324208..4324615 (-) 408 WP_046957547.1 hypothetical protein -
  EXW45_RS22140 (EXW45_22540) - 4324703..4324918 (-) 216 WP_001008334.1 DUF3912 family protein -
  EXW45_RS22145 (EXW45_22545) - 4325173..4325484 (+) 312 WP_001093232.1 hypothetical protein -
  EXW45_RS22150 (EXW45_22550) - 4325521..4326009 (-) 489 WP_000764277.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39295.84 Da        Isoelectric Point: 8.9017

>NTDB_id=346490 EXW45_RS22115 WP_060488444.1 4320163..4321206(-) (comGA) [Bacillus wiedmannii strain JAS08/1]
MNGIEIFANTILKEACRVQASDLHIVPRKKDVVVQLRIGKDLMTKHCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IKKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TSDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCQRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346490 EXW45_RS22115 WP_060488444.1 4320163..4321206(-) (comGA) [Bacillus wiedmannii strain JAS08/1]
ATGAATGGAATTGAAATTTTTGCGAATACGATTTTGAAAGAGGCGTGCAGGGTACAAGCTTCGGATTTACATATTGTGCC
CAGGAAGAAGGATGTAGTGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACACTGTATCGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGACGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAAAAACTACTCTCTTTTTTAC
ACTATTCACATGGGTTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAAAAAAAAGAAAACACGTCGCATCGTTACACTTGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTAAAGGCCATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATCGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACTAGTGATGCGAAAGGGGCGATACTCCGATTTATGGATTTCGGTATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCCAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AGGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCAATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCACGAAACATTAGAATCTTCGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTGTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B5SJ93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.501

100

0.585
  pilB Vibrio campbellii strain DS40M4

36.286

100

0.366

Multiple sequence alignment