Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW45_RS20920 Genome accession   NZ_CP036073
Coordinates   4118204..4118638 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus wiedmannii strain JAS08/1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4113204..4123638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW45_RS20900 (EXW45_21265) - 4113601..4114158 (+) 558 WP_000062072.1 PadR family transcriptional regulator -
  EXW45_RS20905 (EXW45_21270) - 4114350..4114781 (-) 432 WP_046957439.1 NUDIX hydrolase -
  EXW45_RS20910 (EXW45_21275) metE 4115452..4117740 (+) 2289 WP_098091959.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW45_RS20915 (EXW45_21280) comJ 4117778..4118167 (-) 390 WP_000424016.1 competence protein ComJ -
  EXW45_RS20920 (EXW45_21285) nucA/comI 4118204..4118638 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  EXW45_RS20925 (EXW45_21290) - 4118875..4119624 (+) 750 WP_000387495.1 DUF3967 domain-containing protein -
  EXW45_RS20930 (EXW45_21295) kinB 4119677..4120951 (-) 1275 WP_000420378.1 sporulation sensor histidine kinase KinB -
  EXW45_RS20935 (EXW45_21300) - 4121261..4121461 (+) 201 WP_000929285.1 hypothetical protein -
  EXW45_RS20940 (EXW45_21305) - 4121585..4122748 (+) 1164 WP_046957441.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=346487 EXW45_RS20920 WP_000811502.1 4118204..4118638(-) (nucA/comI) [Bacillus wiedmannii strain JAS08/1]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=346487 EXW45_RS20920 WP_000811502.1 4118204..4118638(-) (nucA/comI) [Bacillus wiedmannii strain JAS08/1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCATATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment