Detailed information NTG0346351

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GII89_RS08695
• Genome accession: NZ_CP045812
• Coordinates: 1669730..1670719 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain P8_B3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1664730..1675719

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII89_RS08680 (GII89_08680)	acpP	1664927..1665160 (+)	234	WP_003154310.1	acyl carrier protein	-
GII89_RS08685 (GII89_08685)	rncS	1665300..1666049 (+)	750	WP_003232030.1	ribonuclease III	-
GII89_RS08690 (GII89_08690)	smc	1666150..1669710 (+)	3561	WP_003232028.1	chromosome segregation protein SMC	-
GII89_RS08695 (GII89_08695)	pilA	1669730..1670719 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
GII89_RS08700 (GII89_08700)	sivC	1670756..1671241 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
GII89_RS08705 (GII89_08705)	srpM	1671418..1671750 (+)	333	WP_003238590.1	putative DNA-binding protein	-
GII89_RS08710 (GII89_08710)	ffh	1671764..1673104 (+)	1341	WP_003232020.1	signal recognition particle protein	-
GII89_RS08715 (GII89_08715)	rpsP	1673210..1673482 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
GII89_RS08720 (GII89_08720)	khpA	1673482..1673727 (+)	246	WP_003232017.1	KH domain-containing protein	-
GII89_RS08725 (GII89_08725)	ylqD	1673849..1674235 (+)	387	WP_003232015.1	YlqD family protein	-
GII89_RS08730 (GII89_08730)	rimM	1674240..1674764 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
GII89_RS08735 (GII89_08735)	trmD	1674761..1675492 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=346351 GII89_RS08695 WP_003232026.1 1669730..1670719(+) (pilA) [Bacillus subtilis strain P8_B3]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=346351 GII89_RS08695 WP_003232026.1 1669730..1670719(+) (pilA) [Bacillus subtilis strain P8_B3]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTTGTACTTGCCGCCGGAGACACTTTTAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCAGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401