Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW44_RS19975 Genome accession   NZ_CP036057
Coordinates   3914996..3916039 (-) Length   347 a.a.
NCBI ID   WP_002129093.1    Uniprot ID   A0A1S9TG17
Organism   Bacillus mycoides strain JAS06/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3909996..3921039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW44_RS19930 (EXW44_20185) - 3910394..3910594 (-) 201 WP_002015009.1 YqzE family protein -
  EXW44_RS19935 (EXW44_20190) - 3910633..3911130 (-) 498 WP_215563613.1 shikimate kinase -
  EXW44_RS19940 (EXW44_20195) - 3911282..3911932 (-) 651 WP_002190664.1 2OG-Fe(II) oxygenase -
  EXW44_RS19945 (EXW44_20200) comGG 3912107..3912478 (-) 372 WP_002129085.1 competence type IV pilus minor pilin ComGG -
  EXW44_RS19950 (EXW44_20205) comGF 3912475..3912936 (-) 462 WP_002129086.1 competence type IV pilus minor pilin ComGF -
  EXW44_RS19955 (EXW44_20210) - 3912920..3913216 (-) 297 WP_002129087.1 hypothetical protein -
  EXW44_RS19960 (EXW44_20215) comGD 3913266..3913664 (-) 399 WP_215563615.1 competence type IV pilus minor pilin ComGD -
  EXW44_RS19965 (EXW44_20220) comGC 3913661..3913960 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW44_RS19970 (EXW44_20225) comGB 3913972..3915009 (-) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  EXW44_RS19975 (EXW44_20230) comGA 3914996..3916039 (-) 1044 WP_002129093.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW44_RS19980 (EXW44_20235) - 3916245..3916940 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW44_RS19985 (EXW44_20240) - 3917222..3917464 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW44_RS19990 (EXW44_20245) - 3917572..3918966 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW44_RS19995 (EXW44_20250) - 3919047..3919439 (-) 393 WP_002190672.1 hypothetical protein -
  EXW44_RS20000 (EXW44_20255) - 3919527..3919742 (-) 216 WP_002067106.1 DUF3912 family protein -
  EXW44_RS20005 (EXW44_20260) - 3919995..3920306 (+) 312 WP_002129099.1 hypothetical protein -
  EXW44_RS20010 (EXW44_20265) - 3920342..3920830 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39294.76 Da        Isoelectric Point: 9.4642

>NTDB_id=346337 EXW44_RS19975 WP_002129093.1 3914996..3916039(-) (comGA) [Bacillus mycoides strain JAS06/1]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASAQPLSHLSLFPSSTEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346337 EXW44_RS19975 WP_002129093.1 3914996..3916039(-) (comGA) [Bacillus mycoides strain JAS06/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGCTCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAACGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTAGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S9TG17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment