Detailed information NTG0346304

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: EXW64_RS25555
• Genome accession: NZ_CP036052
• Coordinates: 4964273..4965622 (-)
• Length: 449 a.a.
• NCBI ID: WP_061530594.1
• Uniprot ID: -
• Organism: Bacillus toyonensis strain JAS03/3
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4959273..4970622

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW64_RS25535 (EXW64_26110)	secA	4959602..4962109 (-)	2508	WP_000579396.1	preprotein translocase subunit SecA	-
EXW64_RS25540 (EXW64_26115)	raiA	4962384..4962926 (-)	543	WP_000671188.1	ribosome-associated translation inhibitor RaiA	-
EXW64_RS25545 (EXW64_26120)	cspC	4963245..4963442 (-)	198	WP_002045823.1	cold shock protein CspC	-
EXW64_RS25550 (EXW64_26125)	-	4963569..4964273 (-)	705	WP_000543519.1	ComF family protein	-
EXW64_RS25555 (EXW64_26130)	comFA	4964273..4965622 (-)	1350	WP_061530594.1	ATP-dependent helicase ComFA	Machinery gene
EXW64_RS25560 (EXW64_26135)	-	4965749..4967143 (-)	1395	WP_215589514.1	C40 family peptidase	-
EXW64_RS25565 (EXW64_26140)	-	4967304..4967618 (-)	315	WP_000400852.1	helix-turn-helix domain-containing protein	-
EXW64_RS25570 (EXW64_26145)	-	4967790..4968632 (-)	843	WP_000844774.1	DegV family protein	-
EXW64_RS25575 (EXW64_26150)	-	4968871..4969506 (+)	636	WP_000926674.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 50933.69 Da
• Isoelectric Point: 9.5938

>NTDB_id=346304 EXW64_RS25555 WP_061530594.1 4964273..4965622(-) (comFA) [Bacillus toyonensis strain JAS03/3]
MLSGKQLLLEELSTDVRDNLDDLKKKGEVVCVQGVKNKASTYMCQRCGNIAQRLFSSFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAEKEGEMDVNPLQWKGNLSTGQELAAQGVMEAVKQKESFFIWAVCGAGKTEMLFYGIAEALQKGERVCIAT
PRTDVVLELAPRLKEVFPNINVAALYGGSLDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVKQAM
KEKAACIYLTATPDEEWKRNLRKGKQKGIVVSGRYHRHPLPVPKFCWCGNWKKSLINKRIPQVLLQWLKVYLNKKHPIFL
FVPHVRYIEKMSRLLKLLDDRVAGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGTEEEIFSESALV
QIAGRVGRSFEEPYGEVIYFHYGKTEAMVRAKKHIRSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=346304 EXW64_RS25555 WP_061530594.1 4964273..4965622(-) (comFA) [Bacillus toyonensis strain JAS03/3]
ATGTTATCTGGGAAACAGTTATTATTAGAAGAACTCTCTACAGACGTACGGGATAATTTAGATGATTTGAAGAAGAAGGG
AGAGGTCGTATGTGTACAAGGTGTAAAAAATAAGGCTTCTACATATATGTGTCAGCGTTGCGGAAATATAGCACAAAGAC
TCTTTTCGTCATTTTTGTGTAAAAGGTGTAGTAAAGTCTGCACATATTGCCGGAAGTGCATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTGCCGAAAAAGAAGGAGAAATGGATGTAAATCCGTTACAGTGGAAAGGAAATTT
ATCTACTGGCCAGGAGTTGGCTGCGCAAGGTGTTATGGAGGCTGTTAAACAGAAAGAATCGTTTTTTATTTGGGCTGTGT
GCGGCGCTGGAAAAACAGAAATGTTGTTTTATGGTATTGCAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTACTTGAATTAGCACCGAGATTGAAAGAAGTATTTCCGAATATAAATGTAGCTGCTTTATACGG
TGGAAGTTTAGATCGGGAAAAAGATGCAGCGTTAGTCGTTGCGACCACCCATCAGCTATTGCGTTATTATAGAGCGTTTC
ATGTCATGATTGTAGATGAAATAGATGCCTTTCCATACCATGCAGATCAAATGTTGCAGTATGCGGTAAAACAAGCGATG
AAAGAGAAGGCGGCATGTATTTATTTAACTGCAACTCCAGATGAAGAGTGGAAGCGTAACCTTAGAAAGGGGAAACAAAA
AGGTATCGTTGTTTCTGGACGGTATCACCGTCATCCGTTGCCAGTCCCCAAATTTTGCTGGTGCGGAAACTGGAAGAAAA
GCCTCATCAATAAAAGAATTCCTCAAGTTTTACTACAATGGTTAAAAGTATACTTAAATAAAAAGCACCCCATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAAAAATGAGTCGGTTATTGAAGCTGTTAGACGATAGAGTTGCCGGTGTGCATGC
AGAAGATCCAATGAGAAAAGAAAAAGTTGCAGCTTTCAGAAAGGGAGAAATTCCTTTATTAGTTACAACGACGATTTTGG
AGAGAGGAGTAACGGTGAAAAATTTGCAAGTTGCGGTTTTAGGGACGGAAGAAGAAATATTTTCAGAAAGTGCACTCGTA
CAAATTGCAGGCCGGGTAGGTCGTAGTTTTGAAGAGCCGTATGGAGAGGTCATTTATTTTCATTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACACATTCGAAGTATGAACAAAAATGCGAAAGAACAAGGGTTGATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

• Type III restriction enzyme, res subunit (ResIII) (106-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.679	98.441	0.499
comFA	Latilactobacillus sakei subsp. sakei 23K	40.695	89.755	0.365