Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EXW64_RS21325 Genome accession   NZ_CP036052
Coordinates   4182078..4182824 (-) Length   248 a.a.
NCBI ID   WP_000487005.1    Uniprot ID   A0A2B5BXL0
Organism   Bacillus toyonensis strain JAS03/3     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4177078..4187824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW64_RS21305 (EXW64_21790) dnaG 4177838..4179634 (-) 1797 WP_000528230.1 DNA primase -
  EXW64_RS21310 (EXW64_21795) - 4179888..4180127 (-) 240 WP_002039621.1 DUF188 domain-containing protein -
  EXW64_RS21315 (EXW64_21800) - 4180496..4181308 (-) 813 WP_000368950.1 pyruvate, water dikinase regulatory protein -
  EXW64_RS21320 (EXW64_21805) - 4181338..4181970 (-) 633 WP_000583755.1 helix-turn-helix transcriptional regulator -
  EXW64_RS21325 (EXW64_21810) recO 4182078..4182824 (-) 747 WP_000487005.1 DNA repair protein RecO Machinery gene
  EXW64_RS21330 (EXW64_21815) - 4182858..4183001 (-) 144 WP_000883923.1 YqzL family protein -
  EXW64_RS21335 (EXW64_21820) era 4183174..4184079 (-) 906 WP_001080234.1 GTPase Era -
  EXW64_RS21340 (EXW64_21825) - 4184072..4184470 (-) 399 WP_001086291.1 cytidine deaminase -
  EXW64_RS21345 (EXW64_21830) - 4184569..4184922 (-) 354 WP_000715513.1 diacylglycerol kinase family protein -
  EXW64_RS21350 (EXW64_21835) ybeY 4184919..4185389 (-) 471 WP_000054674.1 rRNA maturation RNase YbeY -
  EXW64_RS21355 (EXW64_21840) - 4185386..4187530 (-) 2145 WP_000091505.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28620.17 Da        Isoelectric Point: 8.0566

>NTDB_id=346294 EXW64_RS21325 WP_000487005.1 4182078..4182824(-) (recO) [Bacillus toyonensis strain JAS03/3]
MFQKVEGIVIRTTDYGETNKIVTIFSREFGKISAMARGAKKPKSRLASVSQLMTHGHFLIQVGSGLGTLQQGEIISTMKE
IREDIFLTAYASFVVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=346294 EXW64_RS21325 WP_000487005.1 4182078..4182824(-) (recO) [Bacillus toyonensis strain JAS03/3]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAATAAGATTGTTACAATATTCTCAAG
AGAATTCGGTAAGATAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGGCATTTTCTTATTCAAGTGGGATCTGGGCTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTGTTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTACTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTATTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCGGAGCAAGATCCGTATCGTATACCGGTGGG
AGAAGCTGTACATAAATTGTTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGCTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTCCTA
GAGCAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B5BXL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

56.275

99.597

0.56

Multiple sequence alignment