Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EXW64_RS05955 Genome accession   NZ_CP036052
Coordinates   1150365..1152191 (+) Length   608 a.a.
NCBI ID   WP_000003362.1    Uniprot ID   A0A2B6VDW5
Organism   Bacillus toyonensis strain JAS03/3     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1145365..1157191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW64_RS05935 (EXW64_06060) - 1145669..1146343 (-) 675 WP_097883134.1 TerC family protein -
  EXW64_RS05940 (EXW64_06065) mecA 1146689..1147372 (+) 684 WP_000402125.1 adaptor protein MecA Regulator
  EXW64_RS05945 (EXW64_06070) - 1147445..1148989 (+) 1545 WP_000799211.1 cardiolipin synthase -
  EXW64_RS05950 (EXW64_06075) - 1149070..1150314 (+) 1245 WP_097895173.1 competence protein CoiA family protein -
  EXW64_RS05955 (EXW64_06080) pepF 1150365..1152191 (+) 1827 WP_000003362.1 oligoendopeptidase F Regulator
  EXW64_RS05960 (EXW64_06090) - 1152727..1153620 (-) 894 WP_000360648.1 ClpXP adapter SpxH family protein -
  EXW64_RS05965 (EXW64_06095) - 1153620..1154018 (-) 399 WP_000043381.1 hypothetical protein -
  EXW64_RS05970 (EXW64_06100) - 1154200..1154778 (-) 579 WP_000191111.1 CYTH domain-containing protein -
  EXW64_RS05975 (EXW64_06105) - 1154923..1155294 (+) 372 WP_001180013.1 hypothetical protein -
  EXW64_RS05980 (EXW64_06110) - 1155325..1155963 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  EXW64_RS05985 (EXW64_06115) - 1155981..1156778 (+) 798 WP_000673191.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70342.69 Da        Isoelectric Point: 4.6476

>NTDB_id=346278 EXW64_RS05955 WP_000003362.1 1150365..1152191(+) (pepF) [Bacillus toyonensis strain JAS03/3]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPQLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQAFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDRRVREDAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDHLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDMLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=346278 EXW64_RS05955 WP_000003362.1 1150365..1152191(+) (pepF) [Bacillus toyonensis strain JAS03/3]
ATGTCTGAACAAAATAAAGCAAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTTCAAGCGATTAAAGAGTTACTACCGCAGTTAACTGAATTTAAAGGGA
AGCTTGGTGACTCTGCGAACAACTTACTTGAGGCACTGCAATATGAAGATGAAATTTCAATGCGACTAGGTAAGCTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCTTTAAATGATCGTGCGACGAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACACTGCAAGCATTTTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGTCCACACGTATTATCTGAGGCT
GAGGAAGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCGTCTATTAAAGGCGAGGACGGAGAAGAAATAGAAATTACGCATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGATGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGAGCGGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCTGCATTGAGCAA
TAATAATATTCCTGAAGCAGTTTACGATCAACTCGTTGAAACGGTAAATGATCACTTACATTTATTGCACCGTTACATCG
ATATTCGAAAGCGTGCGCTAGGACTTGATGAACTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTGAAATATGAAGAAGCACAAGACATGTTATTAAAATCTTTAAACGTACTTGGTGATGAGTATGTTGAAATTTTGAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCTTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCT
GTGCATAGTTATTACACAAGAAAGACACAGCCGCACGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCGTCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGCCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTCGCAGAGTTTGAGCATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAAATTGGGTTAGAATGGTCTCGCATTCCGCACTTCTACTACAACTATTACGTATATCAATATGCAACAG
GATTTAGTGCAGCAACGGCTCTATCTAAACAGATTTTAGAAGAAGGGCAACCAGCGGTAGAACGTTACATTAATGAATTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B6VDW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.747

97.533

0.485

Multiple sequence alignment