Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW28_RS25355 Genome accession   NZ_CP036046
Coordinates   4912039..4913388 (-) Length   449 a.a.
NCBI ID   WP_215585143.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN601     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4907039..4918388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW28_RS25325 (EXW28_25910) - 4907683..4908378 (-) 696 WP_060749253.1 hypothetical protein -
  EXW28_RS25330 (EXW28_25915) - 4908365..4909153 (-) 789 WP_215584246.1 hypothetical protein -
  EXW28_RS25335 (EXW28_25920) - 4909185..4909769 (-) 585 WP_215551422.1 hypothetical protein -
  EXW28_RS25340 (EXW28_25925) raiA 4910148..4910690 (-) 543 WP_002034758.1 ribosome-associated translation inhibitor RaiA -
  EXW28_RS25345 (EXW28_25930) cspC 4911011..4911208 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW28_RS25350 (EXW28_25935) - 4911335..4912039 (-) 705 WP_215584247.1 ComF family protein -
  EXW28_RS25355 (EXW28_25940) comFA 4912039..4913388 (-) 1350 WP_215585143.1 ATP-dependent helicase ComFA Machinery gene
  EXW28_RS25360 (EXW28_25945) - 4913519..4914844 (-) 1326 WP_215584248.1 C40 family peptidase -
  EXW28_RS25365 (EXW28_25950) - 4915109..4915423 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW28_RS25370 (EXW28_25955) - 4915597..4916439 (-) 843 WP_002016143.1 DegV family protein -
  EXW28_RS25375 (EXW28_25960) - 4916832..4917467 (+) 636 WP_002143754.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50913.30 Da        Isoelectric Point: 9.5337

>NTDB_id=346254 EXW28_RS25355 WP_215585143.1 4912039..4913388(-) (comFA) [Bacillus mycoides strain BPN601]
MLAGRQLLLEELSSDLQNKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRTLLQWLNLYLSKRYPIFL
FVPHVRYVEEVSQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=346254 EXW28_RS25355 WP_215585143.1 4912039..4913388(-) (comFA) [Bacillus mycoides strain BPN601]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTGCAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTGCTATGTAAAAGGTGTAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGATACCATCGTCATCCATTGCCAGTTCCAATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGCACTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAGTTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTGCAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.34

98.218

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

41.457

88.641

0.367

Multiple sequence alignment