Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EXW28_RS05965 Genome accession   NZ_CP036046
Coordinates   1194177..1196003 (+) Length   608 a.a.
NCBI ID   WP_002140837.1    Uniprot ID   A0A2A7ZGK4
Organism   Bacillus mycoides strain BPN601     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1189177..1201003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW28_RS05945 (EXW28_06110) - 1189388..1190062 (-) 675 WP_061674935.1 TerC family protein -
  EXW28_RS05950 (EXW28_06115) mecA 1190499..1191182 (+) 684 WP_002011351.1 adaptor protein MecA Regulator
  EXW28_RS05955 (EXW28_06120) - 1191255..1192799 (+) 1545 WP_002064255.1 cardiolipin synthase -
  EXW28_RS05960 (EXW28_06125) - 1192891..1194126 (+) 1236 WP_215584689.1 competence protein CoiA family protein -
  EXW28_RS05965 (EXW28_06130) pepF 1194177..1196003 (+) 1827 WP_002140837.1 oligoendopeptidase F Regulator
  EXW28_RS05970 - 1196136..1196309 (-) 174 WP_000748141.1 hypothetical protein -
  EXW28_RS05975 (EXW28_06135) - 1196540..1197433 (-) 894 WP_002011359.1 ClpXP adapter SpxH family protein -
  EXW28_RS05980 (EXW28_06140) - 1197433..1197831 (-) 399 WP_000043388.1 hypothetical protein -
  EXW28_RS05985 (EXW28_06145) - 1198017..1198595 (-) 579 WP_002064261.1 CYTH domain-containing protein -
  EXW28_RS05990 (EXW28_06150) - 1198740..1199111 (+) 372 WP_002030390.1 hypothetical protein -
  EXW28_RS05995 (EXW28_06155) - 1199142..1199780 (+) 639 WP_002125951.1 GTP pyrophosphokinase family protein -
  EXW28_RS06000 (EXW28_06160) - 1199799..1200596 (+) 798 WP_002011365.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70301.71 Da        Isoelectric Point: 4.6710

>NTDB_id=346227 EXW28_RS05965 WP_002140837.1 1194177..1196003(+) (pepF) [Bacillus mycoides strain BPN601]
MAEKNTVKSLPNRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLQENRELSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDLLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLYTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDKEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=346227 EXW28_RS05965 WP_002140837.1 1194177..1196003(+) (pepF) [Bacillus mycoides strain BPN601]
ATGGCTGAAAAAAATACAGTAAAATCATTACCAAATCGCAATGAGATTGAAGAAGCAAGCACATGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCAAAGTTAACTGAATTTAAAGGGA
AGCTTGGCGACTCTGCAGACAATTTGCTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCACTGAATGATCGCGCAACAAATTTATA
CTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACATTGCAAACATTCTTAC
AAGAAAATCGAGAACTAAGTGTATATGAGCATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCTGAGGCA
GAGGAAGCGTTATTAGCAGAAGCATCTGAAGTGATGAGTGCATCAAGTAATACATTCGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAGGTAGAAATAACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGATGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGGGCAGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCAGCATTAAGCAA
TAATAATATTCCTGAAGCAGTATACGATCAACTCGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATTG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTGAAGTATGAAGAAGCGCAAGACCTGTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGAAATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTACGAGAATAAAGGCAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATATACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTATTACACAAGAAAGACACAGCCACACGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACAATGTTTGCAGAGTTCGAGCATATCATTCATAAAAAAGTACAA
GAAGGACATGCGGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGGGATGCTTTAGT
AATCGATAAAGAAATCGGTTTAGAATGGTCTCGTATTCCGCACTTCTATTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACTGCGTTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTACATTAACGAATTC
TTAAAAGCGGGAAGCTCCGATTATCCAATTGAAGTGTTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTTCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A7ZGK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.916

97.533

0.487

Multiple sequence alignment