Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EXW50_RS21185 Genome accession   NZ_CP036040
Coordinates   4149723..4150469 (-) Length   248 a.a.
NCBI ID   WP_002129136.1    Uniprot ID   A0A366GTN2
Organism   Bacillus mycoides strain BPN573     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4144723..4155469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW50_RS21165 (EXW50_21655) dnaG 4145450..4147252 (-) 1803 WP_002015179.1 DNA primase -
  EXW50_RS29390 - 4147334..4147477 (-) 144 WP_235706372.1 hypothetical protein -
  EXW50_RS21175 (EXW50_21665) - 4148142..4148954 (-) 813 WP_002015180.1 pyruvate, water dikinase regulatory protein -
  EXW50_RS21180 (EXW50_21670) - 4148984..4149616 (-) 633 WP_002015181.1 helix-turn-helix transcriptional regulator -
  EXW50_RS21185 (EXW50_21675) recO 4149723..4150469 (-) 747 WP_002129136.1 DNA repair protein RecO Machinery gene
  EXW50_RS21190 (EXW50_21680) - 4150503..4150646 (-) 144 WP_000883924.1 YqzL family protein -
  EXW50_RS21195 (EXW50_21685) era 4150823..4151728 (-) 906 WP_002088672.1 GTPase Era -
  EXW50_RS21200 (EXW50_21690) - 4151721..4152119 (-) 399 WP_002015184.1 cytidine deaminase -
  EXW50_RS21205 (EXW50_21695) - 4152219..4152572 (-) 354 WP_002129138.1 diacylglycerol kinase family protein -
  EXW50_RS21210 (EXW50_21700) ybeY 4152569..4153039 (-) 471 WP_002088675.1 rRNA maturation RNase YbeY -
  EXW50_RS21215 (EXW50_21705) - 4153036..4155180 (-) 2145 WP_002015188.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28668.23 Da        Isoelectric Point: 8.0540

>NTDB_id=346142 EXW50_RS21185 WP_002129136.1 4149723..4150469(-) (recO) [Bacillus mycoides strain BPN573]
MFQKVEGIVIRTTDYGETNKIVTIYSREFGKVSAMARGAKKPKSRLAAISQLMTHGHFLIQMGSGLGTLQQGEIISSMKE
IREDIFLTAYASFIIELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRTVLNTYYDEYCGIYLKTRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=346142 EXW50_RS21185 WP_002129136.1 4149723..4150469(-) (recO) [Bacillus mycoides strain BPN573]
ATGTTTCAAAAAGTTGAGGGCATTGTTATCCGTACGACAGATTACGGAGAAACAAATAAGATTGTTACAATATACTCACG
GGAATTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCGAAAAAGCCGAAGAGTCGGTTAGCAGCTATTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCATCTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTATTGAATTAACCGATAAAGCAACAGAAGATAAGAAGCA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCTATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCCGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCGGAGCAAGATCCGTATCGTATTCCGGTGGG
AGAGGCTGTTCATAAATTATTACGTCTCTTTTTTCACTTTGATTTACACCGGCTCGGTAATGTATCGGTGAAGGATAGTA
CAAAAAAACAAATGCGCACAGTATTGAATACATATTATGACGAATATTGTGGGATTTATTTGAAAACAAGGCGTTTCTTG
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366GTN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment