Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW49_RS25465 Genome accession   NZ_CP036023
Coordinates   4945011..4946360 (-) Length   449 a.a.
NCBI ID   WP_215588612.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN52/2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4940011..4951360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW49_RS29705 (EXW49_25930) - 4942584..4942697 (-) 114 Protein_4983 YafY family transcriptional regulator -
  EXW49_RS25445 (EXW49_25935) - 4942648..4943016 (+) 369 WP_227590941.1 VOC family protein -
  EXW49_RS25450 (EXW49_25940) raiA 4943120..4943662 (-) 543 WP_002034758.1 ribosome-associated translation inhibitor RaiA -
  EXW49_RS25455 (EXW49_25945) cspC 4943983..4944180 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW49_RS25460 (EXW49_25950) - 4944307..4945011 (-) 705 WP_002129929.1 ComF family protein -
  EXW49_RS25465 (EXW49_25955) comFA 4945011..4946360 (-) 1350 WP_215588612.1 ATP-dependent helicase ComFA Machinery gene
  EXW49_RS25470 (EXW49_25960) - 4946491..4947810 (-) 1320 WP_215588613.1 C40 family peptidase -
  EXW49_RS25475 (EXW49_25965) - 4948075..4948389 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW49_RS25480 (EXW49_25970) - 4948552..4949394 (-) 843 WP_002016143.1 DegV family protein -
  EXW49_RS25485 (EXW49_25975) - 4949633..4950268 (+) 636 WP_215588614.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50837.27 Da        Isoelectric Point: 9.4725

>NTDB_id=346002 EXW49_RS25465 WP_215588612.1 4945011..4946360(-) (comFA) [Bacillus mycoides strain BPN52/2]
MLAGRQLLLEELSSDLQNKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRCPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=346002 EXW49_RS25465 WP_215588612.1 4945011..4946360(-) (comFA) [Bacillus mycoides strain BPN52/2]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTGCAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATGCCCCATTTTTTTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTGCAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(314-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.567

98.218

0.497
  comFA Latilactobacillus sakei subsp. sakei 23K

40.955

88.641

0.363

Multiple sequence alignment