Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW49_RS20735 Genome accession   NZ_CP036023
Coordinates   4071360..4072403 (-) Length   347 a.a.
NCBI ID   WP_215588496.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN52/2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066360..4077403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW49_RS20690 (EXW49_21130) - 4066759..4066959 (-) 201 WP_002015009.1 YqzE family protein -
  EXW49_RS20695 (EXW49_21135) - 4066998..4067495 (-) 498 WP_002129082.1 shikimate kinase -
  EXW49_RS20700 (EXW49_21140) - 4067647..4068297 (-) 651 WP_002088615.1 2OG-Fe(II) oxygenase -
  EXW49_RS20705 (EXW49_21145) comGG 4068471..4068842 (-) 372 WP_061674785.1 competence type IV pilus minor pilin ComGG -
  EXW49_RS20710 (EXW49_21150) comGF 4068839..4069300 (-) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  EXW49_RS20715 (EXW49_21155) - 4069284..4069580 (-) 297 WP_002088621.1 hypothetical protein -
  EXW49_RS20720 (EXW49_21160) comGD 4069573..4070028 (-) 456 WP_002088622.1 competence type IV pilus minor pilin ComGD -
  EXW49_RS20725 (EXW49_21165) comGC 4070025..4070324 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW49_RS20730 (EXW49_21170) comGB 4070336..4071373 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  EXW49_RS20735 (EXW49_21175) comGA 4071360..4072403 (-) 1044 WP_215588496.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW49_RS20740 (EXW49_21180) - 4072609..4073304 (+) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  EXW49_RS20745 (EXW49_21185) - 4073544..4073786 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW49_RS20750 (EXW49_21190) - 4073894..4075288 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW49_RS20755 (EXW49_21195) - 4075369..4075761 (-) 393 Protein_4074 hypothetical protein -
  EXW49_RS20760 (EXW49_21200) - 4075849..4076064 (-) 216 WP_002015115.1 DUF3912 family protein -
  EXW49_RS20765 (EXW49_21205) - 4076317..4076628 (+) 312 WP_002165070.1 hypothetical protein -
  EXW49_RS20770 (EXW49_21210) - 4076666..4077154 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39236.68 Da        Isoelectric Point: 9.3368

>NTDB_id=345991 EXW49_RS20735 WP_215588496.1 4071360..4072403(-) (comGA) [Bacillus mycoides strain BPN52/2]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTAYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345991 EXW49_RS20735 WP_215588496.1 4071360..4072403(-) (comGA) [Bacillus mycoides strain BPN52/2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGCATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GTGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363

Multiple sequence alignment