Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW49_RS19505 Genome accession   NZ_CP036023
Coordinates   3863785..3864219 (-) Length   144 a.a.
NCBI ID   WP_016100690.1    Uniprot ID   R8I4K8
Organism   Bacillus mycoides strain BPN52/2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3858785..3869219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW49_RS19490 (EXW49_19905) - 3859927..3860484 (+) 558 WP_002128898.1 PadR family transcriptional regulator -
  EXW49_RS19495 (EXW49_19910) metE 3861026..3863314 (+) 2289 WP_002014819.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW49_RS19500 (EXW49_19915) comJ 3863356..3863745 (-) 390 WP_002088359.1 competence protein ComJ -
  EXW49_RS19505 (EXW49_19920) nucA/comI 3863785..3864219 (-) 435 WP_016100690.1 DNA-entry nuclease Machinery gene
  EXW49_RS19510 (EXW49_19925) - 3864456..3865199 (+) 744 WP_002033817.1 DUF3967 domain-containing protein -
  EXW49_RS19515 (EXW49_19930) - 3865259..3866533 (-) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  EXW49_RS19520 (EXW49_19935) - 3866843..3867043 (+) 201 WP_000929276.1 hypothetical protein -
  EXW49_RS19525 (EXW49_19940) - 3867278..3868441 (+) 1164 WP_016100689.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16206.64 Da        Isoelectric Point: 8.4331

>NTDB_id=345988 EXW49_RS19505 WP_016100690.1 3863785..3864219(-) (nucA/comI) [Bacillus mycoides strain BPN52/2]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTITYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=345988 EXW49_RS19505 WP_016100690.1 3863785..3864219(-) (nucA/comI) [Bacillus mycoides strain BPN52/2]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAATTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8I4K8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment