Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW52_RS20970 Genome accession   NZ_CP036017
Coordinates   4105093..4106136 (-) Length   347 a.a.
NCBI ID   WP_215600710.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN51/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4100093..4111136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW52_RS20925 (EXW52_21390) - 4100492..4100692 (-) 201 WP_002015009.1 YqzE family protein -
  EXW52_RS20930 (EXW52_21395) - 4100731..4101228 (-) 498 WP_002129082.1 shikimate kinase -
  EXW52_RS20935 (EXW52_21400) - 4101380..4102030 (-) 651 WP_002034016.1 2OG-Fe(II) oxygenase -
  EXW52_RS20940 (EXW52_21405) comGG 4102204..4102575 (-) 372 WP_033709423.1 competence type IV pilus minor pilin ComGG -
  EXW52_RS20945 (EXW52_21410) comGF 4102572..4103033 (-) 462 WP_002165076.1 competence type IV pilus minor pilin ComGF -
  EXW52_RS20950 (EXW52_21415) - 4103017..4103313 (-) 297 WP_016100615.1 hypothetical protein -
  EXW52_RS20955 (EXW52_21420) comGD 4103306..4103761 (-) 456 WP_078184431.1 competence type IV pilus minor pilin ComGD -
  EXW52_RS20960 (EXW52_21425) comGC 4103758..4104057 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW52_RS20965 (EXW52_21430) comGB 4104069..4105106 (-) 1038 WP_321147153.1 competence type IV pilus assembly protein ComGB -
  EXW52_RS20970 (EXW52_21435) comGA 4105093..4106136 (-) 1044 WP_215600710.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW52_RS20975 (EXW52_21440) - 4106342..4107037 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW52_RS20980 (EXW52_21445) - 4107319..4107561 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW52_RS20985 (EXW52_21450) - 4107669..4109063 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW52_RS20990 (EXW52_21455) - 4109144..4109536 (-) 393 WP_016100613.1 hypothetical protein -
  EXW52_RS20995 (EXW52_21460) - 4109624..4109839 (-) 216 WP_002015115.1 DUF3912 family protein -
  EXW52_RS21000 (EXW52_21465) - 4110092..4110403 (+) 312 WP_002165070.1 hypothetical protein -
  EXW52_RS21005 (EXW52_21470) - 4110440..4110928 (-) 489 WP_215600711.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39236.68 Da        Isoelectric Point: 9.3368

>NTDB_id=345940 EXW52_RS20970 WP_215600710.1 4105093..4106136(-) (comGA) [Bacillus mycoides strain BPN51/1]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASAQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345940 EXW52_RS20970 WP_215600710.1 4105093..4106136(-) (comGA) [Bacillus mycoides strain BPN51/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGCTCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGACTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363

Multiple sequence alignment