Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW52_RS05740 Genome accession   NZ_CP036017
Coordinates   1140570..1144295 (+) Length   1241 a.a.
NCBI ID   WP_061688971.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN51/1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1135570..1149295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW52_RS05725 (EXW52_05845) - 1135727..1136320 (+) 594 WP_016119803.1 TVP38/TMEM64 family protein -
  EXW52_RS05730 (EXW52_05850) lepB 1136377..1136940 (+) 564 WP_016119804.1 signal peptidase I -
  EXW52_RS05735 (EXW52_05855) addB 1137058..1140573 (+) 3516 WP_061688972.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW52_RS05740 (EXW52_05860) addA 1140570..1144295 (+) 3726 WP_061688971.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW52_RS05745 (EXW52_05865) - 1144319..1144597 (+) 279 WP_002011271.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW52_RS05750 (EXW52_05870) - 1144813..1145520 (+) 708 WP_060749458.1 DNA alkylation repair protein -
  EXW52_RS05755 (EXW52_05875) gerPF 1145576..1145791 (-) 216 WP_001141570.1 spore germination protein GerPF -
  EXW52_RS05760 (EXW52_05880) - 1145834..1146220 (-) 387 WP_002087037.1 spore germination protein GerPE -
  EXW52_RS05765 (EXW52_05885) gerPD 1146235..1146429 (-) 195 WP_002087035.1 spore germination protein GerPD -
  EXW52_RS05770 (EXW52_05890) - 1146436..1147050 (-) 615 WP_002087034.1 spore germination protein GerPC -
  EXW52_RS05775 (EXW52_05895) gerPB 1147118..1147324 (-) 207 WP_001012506.1 spore germination protein GerPB -
  EXW52_RS05780 (EXW52_05900) gerPA 1147339..1147560 (-) 222 WP_001111190.1 spore germination protein GerPA -
  EXW52_RS05785 (EXW52_05905) - 1147657..1147836 (-) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -
  EXW52_RS05790 (EXW52_05910) - 1148080..1148982 (+) 903 WP_002125896.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142589.82 Da        Isoelectric Point: 4.9021

>NTDB_id=345919 EXW52_RS05740 WP_061688971.1 1140570..1144295(+) (addA) [Bacillus mycoides strain BPN51/1]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEEGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYSASWK
VEVVEGKDLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYADATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFTGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIDE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=345919 EXW52_RS05740 WP_061688971.1 1140570..1144295(+) (addA) [Bacillus mycoides strain BPN51/1]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACAATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAACTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGAGTTGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCTCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAAAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAGGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGAGGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGCGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACTGAGGAAGAAGTGCAAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTCATTGATAAC
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAGGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGTGGTTTACCAGCCGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTGCGCTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCCCTCGGAGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTAACACGT
GCAAAAGAGAAGTTGATTTTAATTGGAACAGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATTCGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGGCGAAATTTATGAATATAGTGCTAGCTGGAAA
GTAGAAGTTGTTGAAGGGAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAATTATTAGAAGCGCTTCG
TGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGGAATACGGGTATGCGG
ACGCGACATCTCACCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAGCGCGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCACAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGAAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.569

100

0.538

Multiple sequence alignment