Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW40_RS25135 Genome accession   NZ_CP036009
Coordinates   4877562..4878911 (-) Length   449 a.a.
NCBI ID   WP_215587068.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN43/2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4872562..4883911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW40_RS25100 (EXW40_25585) - 4872698..4873393 (-) 696 WP_215587066.1 hypothetical protein -
  EXW40_RS25105 - 4873380..4874198 (-) 819 Protein_4915 hypothetical protein -
  EXW40_RS25110 (EXW40_25595) - 4874208..4874769 (-) 562 Protein_4916 hypothetical protein -
  EXW40_RS25115 (EXW40_25600) - 4874888..4875382 (-) 495 WP_215560083.1 hypothetical protein -
  EXW40_RS25120 (EXW40_25605) raiA 4875671..4876213 (-) 543 WP_002089603.1 ribosome-associated translation inhibitor RaiA -
  EXW40_RS25125 (EXW40_25610) cspC 4876534..4876731 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW40_RS25130 (EXW40_25615) - 4876858..4877562 (-) 705 WP_215587067.1 ComF family protein -
  EXW40_RS25135 (EXW40_25620) comFA 4877562..4878911 (-) 1350 WP_215587068.1 ATP-dependent helicase ComFA Machinery gene
  EXW40_RS25140 (EXW40_25625) - 4879042..4880367 (-) 1326 WP_215587069.1 C40 family peptidase -
  EXW40_RS25145 (EXW40_25630) - 4880632..4880946 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW40_RS25150 (EXW40_25635) - 4881109..4881951 (-) 843 WP_002016143.1 DegV family protein -
  EXW40_RS25155 (EXW40_25640) - 4882345..4882980 (+) 636 WP_061689030.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50853.23 Da        Isoelectric Point: 9.4433

>NTDB_id=345851 EXW40_RS25135 WP_215587068.1 4877562..4878911(-) (comFA) [Bacillus mycoides strain BPN43/2]
MLAGRQLLLEELSSDLQNKLDHLKKHREVVCVQGVIKKFSKYTCQRCGNIEQRLFASLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFQWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKSAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=345851 EXW40_RS25135 WP_215587068.1 4877562..4878911(-) (comFA) [Bacillus mycoides strain BPN43/2]
ATGTTAGCTGGTAGACAGTTATTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATTTGAAAAAGCACCG
AGAAGTCGTTTGTGTGCAAGGTGTAATTAAGAAGTTTTCAAAATATACGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGCATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCAGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTTTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTGCAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAGTGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.34

98.218

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365

Multiple sequence alignment