Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   GHR40_RS13465 Genome accession   NZ_CP045741
Coordinates   2843615..2845042 (-) Length   475 a.a.
NCBI ID   WP_077775097.1    Uniprot ID   -
Organism   Escherichia coli strain DH5alpha     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2838615..2850042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHR40_RS13435 (GHR40_13435) yeeZ 2838670..2839494 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  GHR40_RS13440 (GHR40_13440) yeeY 2839540..2840469 (+) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  GHR40_RS13445 (GHR40_13445) yoeI 2840684..2840746 (+) 63 WP_010723108.1 membrane protein YoeI -
  GHR40_RS13450 (GHR40_13450) plaP 2840736..2842094 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  GHR40_RS13455 (GHR40_13455) tsuA 2842273..2843331 (+) 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
  GHR40_RS13460 (GHR40_13460) tsuB 2843345..2843572 (+) 228 WP_046613325.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  GHR40_RS13465 (GHR40_13465) sbcB 2843615..2845042 (-) 1428 WP_077775097.1 exodeoxyribonuclease I Machinery gene
  GHR40_RS13470 (GHR40_13470) dacD 2845251..2846417 (+) 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  GHR40_RS13475 (GHR40_13475) sbmC 2846536..2847009 (+) 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
  GHR40_RS13480 (GHR40_13480) yeeA 2847207..2848265 (+) 1059 WP_046613327.1 FUSC family protein -
  GHR40_RS13485 (GHR40_13485) tmaR 2848437..2848766 (+) 330 WP_000450409.1 DUF496 family protein -
  GHR40_RS13490 (GHR40_13490) - 2848867..2849049 (-) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  GHR40_RS13495 (GHR40_13495) - 2849121..2849249 (+) 129 Protein_2651 transposase domain-containing protein -
  GHR40_RS13500 (GHR40_13500) - 2849538..2849618 (-) 81 Protein_2652 hypothetical protein -
  GHR40_RS13505 (GHR40_13505) yeeW 2849664..2849858 (-) 195 WP_000988600.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54490.78 Da        Isoelectric Point: 5.4700

>NTDB_id=345818 GHR40_RS13465 WP_077775097.1 2843615..2845042(-) (sbcB) [Escherichia coli strain DH5alpha]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLSALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=345818 GHR40_RS13465 WP_077775097.1 2843615..2845042(-) (sbcB) [Escherichia coli strain DH5alpha]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585