Detailed information NTG0345805

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: GHR40_RS09810
• Genome accession: NZ_CP045741
• Coordinates: 2066132..2067124 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081586.1
• Uniprot ID: Q540P1
• Organism: Escherichia coli strain DH5alpha
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2061132..2072124

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GHR40_RS09780 (GHR40_09780)	ispD	2061193..2061903 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
GHR40_RS09785 (GHR40_09785)	ispF	2061903..2062382 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
GHR40_RS09790 (GHR40_09790)	truD	2062379..2063428 (+)	1050	WP_000568970.1	tRNA pseudouridine(13) synthase TruD	-
GHR40_RS09795 (GHR40_09795)	surE	2063409..2064170 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
GHR40_RS09800 (GHR40_09800)	pcm	2064164..2064790 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
GHR40_RS09805 (GHR40_09805)	nlpD	2064975..2066069 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
GHR40_RS09810 (GHR40_09810)	rpoS	2066132..2067124 (+)	993	WP_000081586.1	RNA polymerase sigma factor RpoS	Regulator
GHR40_RS09815 (GHR40_09815)	ygbN	2067218..2068582 (-)	1365	WP_000104441.1	GntP family transporter	-
GHR40_RS09820 (GHR40_09820)	ygbM	2068671..2069447 (-)	777	WP_001136934.1	HPr family phosphocarrier protein	-
GHR40_RS09825 (GHR40_09825)	ygbL	2069452..2070090 (-)	639	WP_001278994.1	aldolase	-
GHR40_RS09830 (GHR40_09830)	otnK	2070182..2071348 (-)	1167	WP_001393459.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37956.89 Da
• Isoelectric Point: 4.5886

>NTDB_id=345805 GHR40_RS09810 WP_000081586.1 2066132..2067124(+) (rpoS) [Escherichia coli strain DH5alpha]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVELEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=345805 GHR40_RS09810 WP_000081586.1 2066132..2067124(+) (rpoS) [Escherichia coli strain DH5alpha]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACTGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: Q540P1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715