Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GHR40_RS06240 Genome accession   NZ_CP045741
Coordinates   1329570..1331063 (+) Length   497 a.a.
NCBI ID   WP_001040654.1    Uniprot ID   P10121
Organism   Escherichia coli strain DH5alpha     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1324570..1336063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHR40_RS06210 (GHR40_06210) tusA 1324817..1325062 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  GHR40_RS06215 (GHR40_06215) zntA 1325164..1327362 (-) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GHR40_RS06220 (GHR40_06220) yhhN 1327436..1328062 (-) 627 WP_000964718.1 lysoplasmalogenase -
  GHR40_RS06225 (GHR40_06225) yhhM 1328203..1328562 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  GHR40_RS06230 (GHR40_06230) yhhL 1328565..1328834 (-) 270 WP_001311191.1 DUF1145 family protein -
  GHR40_RS06235 (GHR40_06235) rsmD 1328824..1329420 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GHR40_RS06240 (GHR40_06240) pilA 1329570..1331063 (+) 1494 WP_001040654.1 signal recognition particle-docking protein FtsY Machinery gene
  GHR40_RS06245 (GHR40_06245) ftsE 1331066..1331734 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GHR40_RS06250 (GHR40_06250) ftsX 1331727..1332785 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  GHR40_RS06255 (GHR40_06255) rpoH 1333030..1333884 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GHR40_RS06260 (GHR40_06260) livJ 1334155..1335258 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GHR40_RS06265 (GHR40_06265) panM 1335446..1335829 (-) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54513.35 Da        Isoelectric Point: 4.1560

>NTDB_id=345790 GHR40_RS06240 WP_001040654.1 1329570..1331063(+) (pilA) [Escherichia coli strain DH5alpha]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=345790 GHR40_RS06240 WP_001040654.1 1329570..1331063(+) (pilA) [Escherichia coli strain DH5alpha]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1FTS
  PDB 2QY9
  PDB 2XXA
  PDB 2YHS
  PDB 3ZN8
  PDB 4C7O
  PDB 5GAD
  PDB 5NCO
  PDB 5NIY
  PDB 6CQP
  PDB 6CS8
  PDB 6CVD
  PDB 6DLX
  PDB 6FPK
  PDB 6FPR
  PDB 6FQD
  PDB 6N5I
  PDB 6N5J
  PDB 6N6N
  PDB 6N9B
  PDB 6NC1
  PDB 6NC4
  PDB 7O9H

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.698

77.264

0.376