Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW61_RS04765 Genome accession   NZ_CP036004
Coordinates   884492..885535 (+) Length   347 a.a.
NCBI ID   WP_002138337.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN37/2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879492..890535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW61_RS04730 (EXW61_04820) - 879701..880189 (+) 489 WP_002015119.1 hypothetical protein -
  EXW61_RS04735 (EXW61_04825) - 880225..880536 (-) 312 WP_002165070.1 hypothetical protein -
  EXW61_RS04740 (EXW61_04830) - 880789..881004 (+) 216 WP_215599349.1 DUF3912 family protein -
  EXW61_RS04745 (EXW61_04835) - 881092..881484 (+) 393 WP_002190672.1 hypothetical protein -
  EXW61_RS04750 (EXW61_04840) - 881565..882959 (-) 1395 WP_002138338.1 L-cystine transporter -
  EXW61_RS04755 (EXW61_04845) - 883067..883309 (-) 243 WP_002088627.1 DUF2626 domain-containing protein -
  EXW61_RS04760 (EXW61_04850) - 883591..884286 (-) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW61_RS04765 (EXW61_04855) comGA 884492..885535 (+) 1044 WP_002138337.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW61_RS04770 (EXW61_04860) comGB 885522..886559 (+) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  EXW61_RS04775 (EXW61_04865) comGC 886571..886870 (+) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW61_RS04780 (EXW61_04870) comGD 886867..887322 (+) 456 WP_078184431.1 competence type IV pilus minor pilin ComGD -
  EXW61_RS04785 (EXW61_04875) - 887315..887611 (+) 297 WP_016100615.1 hypothetical protein -
  EXW61_RS04790 (EXW61_04880) comGF 887595..888056 (+) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  EXW61_RS04795 (EXW61_04885) comGG 888053..888424 (+) 372 WP_098167362.1 competence type IV pilus minor pilin ComGG -
  EXW61_RS04800 (EXW61_04890) - 888598..889248 (+) 651 WP_098167363.1 2OG-Fe(II) oxygenase -
  EXW61_RS04805 (EXW61_04895) - 889400..889897 (+) 498 WP_002129082.1 shikimate kinase -
  EXW61_RS04810 (EXW61_04900) - 889936..890136 (+) 201 WP_002015009.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39093.53 Da        Isoelectric Point: 9.3369

>NTDB_id=345764 EXW61_RS04765 WP_002138337.1 884492..885535(+) (comGA) [Bacillus mycoides strain BPN37/2]
MNSVELFANMIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKGFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345764 EXW61_RS04765 WP_002138337.1 884492..885535(+) (comGA) [Bacillus mycoides strain BPN37/2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAAAAGGGATTTGGAG
AAAAGCTTGTTTCGCACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGGCAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTAGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.061

100

0.571
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment