Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GHR40_RS00895 Genome accession   NZ_CP045741
Coordinates   173588..174028 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain DH5alpha     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168588..179028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHR40_RS00875 (GHR40_00875) aroP 169586..170959 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GHR40_RS00880 (GHR40_00880) ampE 171002..171856 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GHR40_RS00885 (GHR40_00885) ampD 171853..172404 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GHR40_RS00890 (GHR40_00890) nadC 172492..173385 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GHR40_RS00895 (GHR40_00895) pilA 173588..174028 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GHR40_RS00900 (GHR40_00900) pilB 174038..175423 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  GHR40_RS00905 (GHR40_00905) hofC 175413..176615 (+) 1203 WP_000157266.1 protein transport protein HofC -
  GHR40_RS00910 (GHR40_00910) guaC 176650..177693 (-) 1044 Protein_176 GMP reductase -
  GHR40_RS23360 - 177849..177893 (-) 45 WP_120795372.1 protein YacM -
  GHR40_RS00915 (GHR40_00915) coaE 177918..178538 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=345764 GHR40_RS00895 WP_000360904.1 173588..174028(+) (pilA) [Escherichia coli strain DH5alpha]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=345764 GHR40_RS00895 WP_000360904.1 173588..174028(+) (pilA) [Escherichia coli strain DH5alpha]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment