Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW27_RS21100 Genome accession   NZ_CP035999
Coordinates   4109749..4110792 (+) Length   347 a.a.
NCBI ID   WP_078203155.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN37/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4104749..4115792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW27_RS21065 (EXW27_21440) - 4104958..4105446 (+) 489 WP_002015119.1 hypothetical protein -
  EXW27_RS21070 (EXW27_21445) - 4105483..4105794 (-) 312 WP_002129099.1 hypothetical protein -
  EXW27_RS21075 (EXW27_21450) - 4106047..4106262 (+) 216 WP_002067106.1 DUF3912 family protein -
  EXW27_RS21080 (EXW27_21455) - 4106350..4106742 (+) 393 WP_002190672.1 hypothetical protein -
  EXW27_RS21085 (EXW27_21460) - 4106823..4108217 (-) 1395 WP_147740080.1 L-cystine transporter -
  EXW27_RS21090 (EXW27_21465) - 4108325..4108567 (-) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW27_RS21095 (EXW27_21470) - 4108848..4109543 (-) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW27_RS21100 (EXW27_21475) comGA 4109749..4110792 (+) 1044 WP_078203155.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW27_RS21105 (EXW27_21480) comGB 4110779..4111816 (+) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  EXW27_RS21110 (EXW27_21485) comGC 4111828..4112127 (+) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW27_RS21115 (EXW27_21490) comGD 4112124..4112579 (+) 456 WP_078178172.1 competence type IV pilus minor pilin ComGD -
  EXW27_RS21120 (EXW27_21495) - 4112572..4112868 (+) 297 WP_002088621.1 hypothetical protein -
  EXW27_RS21125 (EXW27_21500) comGF 4112852..4113313 (+) 462 WP_147740079.1 competence type IV pilus minor pilin ComGF -
  EXW27_RS21130 (EXW27_21505) comGG 4113310..4113681 (+) 372 WP_002167676.1 competence type IV pilus minor pilin ComGG -
  EXW27_RS21135 (EXW27_21510) - 4113856..4114506 (+) 651 WP_002167677.1 2OG-Fe(II) oxygenase -
  EXW27_RS21140 (EXW27_21515) - 4114658..4115155 (+) 498 WP_002167678.1 shikimate kinase -
  EXW27_RS21145 (EXW27_21520) - 4115194..4115394 (+) 201 WP_002015009.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39292.79 Da        Isoelectric Point: 9.4642

>NTDB_id=345745 EXW27_RS21100 WP_078203155.1 4109749..4110792(+) (comGA) [Bacillus mycoides strain BPN37/1]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345745 EXW27_RS21100 WP_078203155.1 4109749..4110792(+) (comGA) [Bacillus mycoides strain BPN37/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATTATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment