Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW63_RS20190 Genome accession   NZ_CP035997
Coordinates   3965971..3967014 (+) Length   347 a.a.
NCBI ID   WP_002138337.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN36/3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3960971..3972014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW63_RS20155 (EXW63_20590) - 3961181..3961669 (+) 489 WP_002015119.1 hypothetical protein -
  EXW63_RS20160 (EXW63_20595) - 3961705..3962016 (-) 312 WP_002129099.1 hypothetical protein -
  EXW63_RS20165 (EXW63_20600) - 3962269..3962484 (+) 216 WP_002015115.1 DUF3912 family protein -
  EXW63_RS20170 (EXW63_20605) - 3962554..3962964 (+) 411 WP_165689457.1 hypothetical protein -
  EXW63_RS20175 (EXW63_20610) - 3963044..3964438 (-) 1395 WP_215551695.1 L-cystine transporter -
  EXW63_RS20180 (EXW63_20615) - 3964546..3964788 (-) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW63_RS20185 (EXW63_20620) - 3965070..3965765 (-) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  EXW63_RS20190 (EXW63_20625) comGA 3965971..3967014 (+) 1044 WP_002138337.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW63_RS20195 (EXW63_20630) comGB 3967001..3968038 (+) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  EXW63_RS20200 (EXW63_20635) comGC 3968050..3968349 (+) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW63_RS20205 (EXW63_20640) comGD 3968346..3968801 (+) 456 WP_078184431.1 competence type IV pilus minor pilin ComGD -
  EXW63_RS20210 (EXW63_20645) - 3968794..3969090 (+) 297 WP_215553147.1 type II secretion system protein -
  EXW63_RS20215 (EXW63_20650) comGF 3969074..3969535 (+) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  EXW63_RS20220 (EXW63_20655) comGG 3969532..3969903 (+) 372 WP_113734228.1 competence type IV pilus minor pilin ComGG -
  EXW63_RS20225 (EXW63_20660) - 3970077..3970727 (+) 651 WP_215551696.1 2OG-Fe(II) oxygenase -
  EXW63_RS20230 (EXW63_20665) - 3970879..3971376 (+) 498 WP_002129082.1 shikimate kinase -
  EXW63_RS20235 (EXW63_20670) - 3971415..3971615 (+) 201 WP_002015009.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39093.53 Da        Isoelectric Point: 9.3369

>NTDB_id=345694 EXW63_RS20190 WP_002138337.1 3965971..3967014(+) (comGA) [Bacillus mycoides strain BPN36/3]
MNSVELFANMIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKGFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345694 EXW63_RS20190 WP_002138337.1 3965971..3967014(+) (comGA) [Bacillus mycoides strain BPN36/3]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAAAAGGGATTTGGAG
AAAAGCTTGTTTCGCACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTGCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTAGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGACATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.061

100

0.571
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment