Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW32_RS25635 Genome accession   NZ_CP035994
Coordinates   5014174..5015523 (-) Length   449 a.a.
NCBI ID   WP_215586090.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN36/2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5009174..5020523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW32_RS25600 (EXW32_26145) - 5009295..5009990 (-) 696 WP_002129924.1 hypothetical protein -
  EXW32_RS25605 (EXW32_26150) - 5009977..5010765 (-) 789 WP_002129925.1 hypothetical protein -
  EXW32_RS25610 (EXW32_26155) - 5010797..5011381 (-) 585 WP_002068272.1 hypothetical protein -
  EXW32_RS25615 (EXW32_26160) - 5011500..5011994 (-) 495 WP_215585653.1 hypothetical protein -
  EXW32_RS25620 (EXW32_26165) raiA 5012283..5012825 (-) 543 WP_002089603.1 ribosome-associated translation inhibitor RaiA -
  EXW32_RS25625 (EXW32_26170) cspC 5013146..5013343 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW32_RS25630 (EXW32_26175) - 5013470..5014174 (-) 705 WP_002129929.1 ComF family protein -
  EXW32_RS25635 (EXW32_26180) comFA 5014174..5015523 (-) 1350 WP_215586090.1 ATP-dependent helicase ComFA Machinery gene
  EXW32_RS25640 (EXW32_26185) - 5015654..5016997 (-) 1344 WP_002129932.1 C40 family peptidase -
  EXW32_RS25645 (EXW32_26190) - 5017231..5017545 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW32_RS25650 (EXW32_26195) - 5017708..5018550 (-) 843 WP_002016143.1 DegV family protein -
  EXW32_RS25655 (EXW32_26200) - 5018789..5019424 (+) 636 WP_002034752.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50941.36 Da        Isoelectric Point: 9.4432

>NTDB_id=345645 EXW32_RS25635 WP_215586090.1 5014174..5015523(-) (comFA) [Bacillus mycoides strain BPN36/2]
MLAGRQLLLEELSSDLQDKLDHLKKHREVVCMQGVIKKFSKYTCQRCGNIEQRLFASLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQVDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTIKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=345645 EXW32_RS25635 WP_215586090.1 5014174..5015523(-) (comFA) [Bacillus mycoides strain BPN36/2]
ATGTTAGCTGGTAGACAGTTATTGTTAGAAGAACTCTCTTCAGATTTGCAGGATAAATTGGATCATTTGAAAAAGCACCG
AGAAGTCGTTTGTATGCAAGGTGTAATTAAGAAGTTTTCAAAATATACGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGCATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAGTTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGGGGAGTAACTATTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-409)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.226

98.441

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

40.955

88.641

0.363

Multiple sequence alignment