Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW32_RS20905 Genome accession   NZ_CP035994
Coordinates   4136414..4137457 (-) Length   347 a.a.
NCBI ID   WP_002184965.1    Uniprot ID   A0AAP8H1U1
Organism   Bacillus mycoides strain BPN36/2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4131414..4142457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW32_RS20860 (EXW32_21330) - 4131812..4132012 (-) 201 WP_002015009.1 YqzE family protein -
  EXW32_RS20865 (EXW32_21335) - 4132051..4132548 (-) 498 WP_002167678.1 shikimate kinase -
  EXW32_RS20870 (EXW32_21340) - 4132700..4133350 (-) 651 WP_002167677.1 2OG-Fe(II) oxygenase -
  EXW32_RS20875 (EXW32_21345) comGG 4133525..4133896 (-) 372 WP_002129085.1 competence type IV pilus minor pilin ComGG -
  EXW32_RS20880 (EXW32_21350) comGF 4133893..4134354 (-) 462 WP_002129086.1 competence type IV pilus minor pilin ComGF -
  EXW32_RS20885 (EXW32_21355) - 4134338..4134634 (-) 297 WP_002129087.1 hypothetical protein -
  EXW32_RS20890 (EXW32_21360) comGD 4134627..4135082 (-) 456 WP_002167675.1 competence type IV pilus minor pilin ComGD -
  EXW32_RS20895 (EXW32_21365) comGC 4135079..4135378 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW32_RS20900 (EXW32_21370) comGB 4135390..4136427 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  EXW32_RS20905 (EXW32_21375) comGA 4136414..4137457 (-) 1044 WP_002184965.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW32_RS20910 (EXW32_21380) - 4137663..4138358 (+) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  EXW32_RS20915 (EXW32_21385) - 4138640..4138882 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW32_RS20920 (EXW32_21390) - 4138990..4140384 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW32_RS20925 (EXW32_21395) - 4140465..4140857 (-) 393 WP_002190672.1 hypothetical protein -
  EXW32_RS20930 (EXW32_21400) - 4140945..4141160 (-) 216 WP_002067106.1 DUF3912 family protein -
  EXW32_RS20935 (EXW32_21405) - 4141413..4141724 (+) 312 WP_215585564.1 hypothetical protein -
  EXW32_RS20940 (EXW32_21410) - 4141761..4142249 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39278.81 Da        Isoelectric Point: 9.4873

>NTDB_id=345634 EXW32_RS20905 WP_002184965.1 4136414..4137457(-) (comGA) [Bacillus mycoides strain BPN36/2]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVHQESLVIRLHLQVSVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLEASVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345634 EXW32_RS20905 WP_002184965.1 4136414..4137457(-) (comGA) [Bacillus mycoides strain BPN36/2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTACATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGTATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATTATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTGCGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTGTGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAAGCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573

Multiple sequence alignment