Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW32_RS19715 Genome accession   NZ_CP035994
Coordinates   3932552..3932986 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN36/2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3927552..3937986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW32_RS19700 (EXW32_20145) - 3928696..3929253 (+) 558 WP_078203120.1 PadR family transcriptional regulator -
  EXW32_RS19705 (EXW32_20150) metE 3929794..3932082 (+) 2289 WP_002014819.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW32_RS19710 (EXW32_20155) comJ 3932124..3932513 (-) 390 WP_002088359.1 competence protein ComJ -
  EXW32_RS19715 (EXW32_20160) nucA/comI 3932552..3932986 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  EXW32_RS19720 (EXW32_20165) - 3933223..3933966 (+) 744 WP_002014816.1 DUF3967 domain-containing protein -
  EXW32_RS19725 (EXW32_20170) - 3934026..3935300 (-) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  EXW32_RS19730 (EXW32_20175) - 3935610..3935810 (+) 201 WP_000929276.1 hypothetical protein -
  EXW32_RS19735 (EXW32_20180) - 3936045..3937208 (+) 1164 WP_002128899.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=345631 EXW32_RS19715 WP_002014817.1 3932552..3932986(-) (nucA/comI) [Bacillus mycoides strain BPN36/2]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=345631 EXW32_RS19715 WP_002014817.1 3932552..3932986(-) (nucA/comI) [Bacillus mycoides strain BPN36/2]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment