Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   EXW32_RS07835 Genome accession   NZ_CP035994
Coordinates   1527724..1528326 (-) Length   200 a.a.
NCBI ID   WP_000155584.1    Uniprot ID   J8ATF7
Organism   Bacillus mycoides strain BPN36/2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1522724..1533326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW32_RS07815 (EXW32_07965) dnaD 1523009..1523716 (+) 708 WP_000728546.1 DNA replication protein DnaD -
  EXW32_RS07820 (EXW32_07970) nth 1523732..1524379 (+) 648 WP_000933020.1 endonuclease III -
  EXW32_RS07825 (EXW32_07975) - 1524383..1524901 (+) 519 WP_033730526.1 YpoC family protein -
  EXW32_RS07830 (EXW32_07980) - 1524925..1527657 (-) 2733 WP_215585884.1 PBP1A family penicillin-binding protein -
  EXW32_RS07835 (EXW32_07985) recU 1527724..1528326 (-) 603 WP_000155584.1 Holliday junction resolvase RecU Machinery gene
  EXW32_RS07840 (EXW32_07990) - 1528430..1529455 (+) 1026 WP_002126479.1 DUF2515 domain-containing protein -
  EXW32_RS30290 - 1529799..1529945 (-) 147 WP_307838604.1 phage portal protein -
  EXW32_RS07845 (EXW32_07995) - 1530064..1530306 (+) 243 WP_002011865.1 hypothetical protein -
  EXW32_RS07850 (EXW32_08000) - 1530322..1530666 (+) 345 WP_002126482.1 YppE family protein -
  EXW32_RS07855 (EXW32_08005) - 1530692..1530883 (-) 192 WP_002030817.1 YppF family protein -
  EXW32_RS07860 (EXW32_08010) - 1531061..1531675 (+) 615 WP_002189013.1 YppG family protein -
  EXW32_RS07865 (EXW32_08015) - 1531832..1532269 (+) 438 WP_002168778.1 CotD family spore coat protein -
  EXW32_RS07870 (EXW32_08020) - 1532326..1532880 (+) 555 WP_002030808.1 DUF1273 domain-containing protein -
  EXW32_RS07875 (EXW32_08025) gpsB 1532978..1533313 (+) 336 WP_000622426.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23361.62 Da        Isoelectric Point: 9.5601

>NTDB_id=345621 EXW32_RS07835 WP_000155584.1 1527724..1528326(-) (recU) [Bacillus mycoides strain BPN36/2]
MTIRYPNGKRYDQASQPHKTPIKKHTYSNRGMSLEEELNETNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKQEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=345621 EXW32_RS07835 WP_000155584.1 1527724..1528326(-) (recU) [Bacillus mycoides strain BPN36/2]
ATGACCATTCGTTACCCAAATGGAAAAAGGTACGATCAAGCTTCACAACCTCATAAAACACCAATAAAAAAACATACTTA
CAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACCAATCAATATTACTTAACCCATAATATTGCATGCGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTC
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACTAAAAATAA
AACCAGTTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAGTCGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGTAAATCGATTACAAAACAAGAAATAGAAGAGCATGGGTCTTTATTATCATGCGGTTATCACCCTCG
AATTGATTATATCCGCGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8ATF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63

100

0.63

Multiple sequence alignment