Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EXW32_RS06070 Genome accession   NZ_CP035994
Coordinates   1202013..1203839 (+) Length   608 a.a.
NCBI ID   WP_002134970.1    Uniprot ID   A0A0B5S4T9
Organism   Bacillus mycoides strain BPN36/2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1197013..1208839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW32_RS06050 (EXW32_06195) - 1197224..1197898 (-) 675 WP_002030376.1 TerC family protein -
  EXW32_RS06055 (EXW32_06200) mecA 1198335..1199018 (+) 684 WP_002011351.1 adaptor protein MecA Regulator
  EXW32_RS06060 (EXW32_06205) - 1199091..1200635 (+) 1545 WP_002064255.1 cardiolipin synthase -
  EXW32_RS06065 (EXW32_06210) - 1200727..1201962 (+) 1236 WP_215585861.1 competence protein CoiA family protein -
  EXW32_RS06070 (EXW32_06215) pepF 1202013..1203839 (+) 1827 WP_002134970.1 oligoendopeptidase F Regulator
  EXW32_RS06075 - 1203972..1204145 (-) 174 WP_000748141.1 hypothetical protein -
  EXW32_RS06080 (EXW32_06220) - 1204375..1205268 (-) 894 WP_199626861.1 ClpXP adapter SpxH family protein -
  EXW32_RS06085 (EXW32_06225) - 1205268..1205666 (-) 399 WP_000043388.1 hypothetical protein -
  EXW32_RS06090 (EXW32_06230) - 1205852..1206430 (-) 579 WP_199626860.1 CYTH domain-containing protein -
  EXW32_RS06095 (EXW32_06235) - 1206575..1206946 (+) 372 WP_002011362.1 hypothetical protein -
  EXW32_RS06100 (EXW32_06240) - 1206977..1207615 (+) 639 WP_002125951.1 GTP pyrophosphokinase family protein -
  EXW32_RS06105 (EXW32_06245) - 1207634..1208431 (+) 798 WP_002011365.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70331.69 Da        Isoelectric Point: 4.6480

>NTDB_id=345618 EXW32_RS06070 WP_002134970.1 1202013..1203839(+) (pepF) [Bacillus mycoides strain BPN36/2]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLQENRELSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDLLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLYTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDKEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=345618 EXW32_RS06070 WP_002134970.1 1202013..1203839(+) (pepF) [Bacillus mycoides strain BPN36/2]
ATGTCTGAACAAAATAAAGCAAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGCACATGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCAAAGTTAACTGAATTTAAAGGGA
AGCTTGGCGACTCTGCAGACAATTTGCTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCACTGAATGATCGCGCAACAAATTTATA
CTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACATTGCAAACATTTTTAC
AAGAAAATCGAGAACTAAGTGTATATGAGCATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCTGAGGCA
GAGGAAGCGTTATTAGCAGAAGCATCTGAAGTGATGAGTGCATCAAGTAATACATTCGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAGGTAGAAATAACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGATGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGGGCAGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCAGCATTAAGCAA
TAATAATATTCCTGAAGCAGTATACGATCAACTCGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATTG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTGAAGTATGAAGAAGCGCAAGACCTGTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGAAATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTACGAGAATAAAGGCAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATATACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTATTACACAAGAAAGACACAGCCACACGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACAATGTTTGCAGAGTTCGAGCATATCATTCATAAAAAAGTACAA
GAAGGACATGCGGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGGGATGCTTTAGT
AATCGATAAAGAAATCGGTTTAGAATGGTCTCGTATTCCGCACTTCTATTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACTGCGTTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTACATTAATGAATTC
TTAAAAGCGGGAAGCTCCGATTATCCAATTGAAGTGTTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTTCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5S4T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.747

97.533

0.485

Multiple sequence alignment