Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW60_RS04440 Genome accession   NZ_CP035987
Coordinates   863213..864256 (-) Length   347 a.a.
NCBI ID   WP_002138337.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN29/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858213..869256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW60_RS04395 (EXW60_04480) - 858612..858812 (-) 201 WP_002015009.1 YqzE family protein -
  EXW60_RS04400 (EXW60_04485) - 858851..859348 (-) 498 WP_215586843.1 shikimate kinase -
  EXW60_RS04405 (EXW60_04490) - 859500..860150 (-) 651 WP_002034016.1 2OG-Fe(II) oxygenase -
  EXW60_RS04410 (EXW60_04495) comGG 860324..860695 (-) 372 WP_033709423.1 competence type IV pilus minor pilin ComGG -
  EXW60_RS04415 (EXW60_04500) comGF 860692..861153 (-) 462 WP_061689623.1 competence type IV pilus minor pilin ComGF -
  EXW60_RS04420 (EXW60_04505) - 861137..861433 (-) 297 WP_016100615.1 hypothetical protein -
  EXW60_RS04425 (EXW60_04510) comGD 861426..861881 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  EXW60_RS04430 (EXW60_04515) comGC 861878..862177 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW60_RS04435 (EXW60_04520) comGB 862189..863226 (-) 1038 WP_286673852.1 competence type IV pilus assembly protein ComGB -
  EXW60_RS04440 (EXW60_04525) comGA 863213..864256 (-) 1044 WP_002138337.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW60_RS04445 (EXW60_04530) - 864462..865157 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW60_RS04450 (EXW60_04535) - 865376..865618 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW60_RS04455 (EXW60_04540) - 865726..867120 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW60_RS04460 (EXW60_04545) - 867201..867611 (-) 411 WP_165689457.1 hypothetical protein -
  EXW60_RS04465 (EXW60_04550) - 867681..867896 (-) 216 WP_002015115.1 DUF3912 family protein -
  EXW60_RS04470 (EXW60_04555) - 868149..868460 (+) 312 WP_002129099.1 hypothetical protein -
  EXW60_RS04475 (EXW60_04560) - 868495..868983 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39093.53 Da        Isoelectric Point: 9.3369

>NTDB_id=345551 EXW60_RS04440 WP_002138337.1 863213..864256(-) (comGA) [Bacillus mycoides strain BPN29/1]
MNSVELFANMIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKGFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345551 EXW60_RS04440 WP_002138337.1 863213..864256(-) (comGA) [Bacillus mycoides strain BPN29/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAAAAGGGATTTGGAG
AAAAGCTTGTTTCGCACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTAGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.061

100

0.571
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment