Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW60_RS03160 Genome accession   NZ_CP035987
Coordinates   642827..643261 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN29/1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 637827..648261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW60_RS03145 (EXW60_03210) - 638969..639526 (+) 558 WP_215586791.1 PadR family transcriptional regulator -
  EXW60_RS03150 (EXW60_03215) metE 640068..642356 (+) 2289 WP_215586792.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW60_RS03155 (EXW60_03220) comJ 642398..642787 (-) 390 WP_002088359.1 competence protein ComJ -
  EXW60_RS03160 (EXW60_03225) nucA/comI 642827..643261 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  EXW60_RS03165 (EXW60_03230) - 643498..644241 (+) 744 WP_002033817.1 DUF3967 domain-containing protein -
  EXW60_RS03170 (EXW60_03235) - 644301..645575 (-) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  EXW60_RS03175 (EXW60_03240) - 645885..646085 (+) 201 WP_000929276.1 hypothetical protein -
  EXW60_RS03180 (EXW60_03245) - 646320..647483 (+) 1164 WP_098635987.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=345548 EXW60_RS03160 WP_002014817.1 642827..643261(-) (nucA/comI) [Bacillus mycoides strain BPN29/1]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=345548 EXW60_RS03160 WP_002014817.1 642827..643261(-) (nucA/comI) [Bacillus mycoides strain BPN29/1]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCAGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment