Detailed information NTG0345472

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GHT02_RS14525
• Genome accession: NZ_CP045712
• Coordinates: 3024768..3025751 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain FHI_NMBU_11
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 3019768..3030751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GHT02_RS14510 (GHT02_14485)	dppA	3021068..3022675 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
GHT02_RS14515 (GHT02_14490)	dppB	3022826..3023845 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GHT02_RS14520 (GHT02_14495)	dppC	3023855..3024757 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GHT02_RS14525 (GHT02_14500)	amiE	3024768..3025751 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GHT02_RS14530 (GHT02_14505)	dppF	3025748..3026752 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GHT02_RS14535 (GHT02_14510)	yhjV	3026782..3028053 (-)	1272	WP_001296805.1	aromatic amino acid transport family protein	-
GHT02_RS14540 (GHT02_14515)	-	3028529..3028636 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GHT02_RS14545 (GHT02_14520)	-	3029013..3029120 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GHT02_RS14550 (GHT02_14525)	-	3029496..3029603 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=345472 GHT02_RS14525 WP_001196486.1 3024768..3025751(+) (amiE) [Escherichia coli strain FHI_NMBU_11]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=345472 GHT02_RS14525 WP_001196486.1 3024768..3025751(+) (amiE) [Escherichia coli strain FHI_NMBU_11]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404