Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GHT02_RS08375 Genome accession   NZ_CP045712
Coordinates   1700312..1700752 (-) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain FHI_NMBU_11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1695312..1705752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHT02_RS08350 (GHT02_08355) coaE 1695801..1696421 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  GHT02_RS08355 - 1696446..1696490 (+) 45 WP_120795372.1 protein YacM -
  GHT02_RS08360 (GHT02_08360) guaC 1696646..1697689 (+) 1044 WP_001217338.1 GMP reductase -
  GHT02_RS08365 (GHT02_08365) hofC 1697724..1698926 (-) 1203 WP_000157233.1 protein transport protein HofC -
  GHT02_RS08370 (GHT02_08370) gspE 1698916..1700302 (-) 1387 Protein_1662 type II secretion system protein GspE -
  GHT02_RS08375 (GHT02_08375) pilA 1700312..1700752 (-) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  GHT02_RS08380 (GHT02_08380) nadC 1700955..1701848 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GHT02_RS08385 (GHT02_08385) ampD 1701936..1702487 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GHT02_RS08390 (GHT02_08390) ampE 1702484..1703338 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GHT02_RS08395 (GHT02_08395) aroP 1703381..1704754 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=345446 GHT02_RS08375 WP_000360894.1 1700312..1700752(-) (pilA) [Escherichia coli strain FHI_NMBU_11]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=345446 GHT02_RS08375 WP_000360894.1 1700312..1700752(-) (pilA) [Escherichia coli strain FHI_NMBU_11]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment