Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW31_RS25035 Genome accession   NZ_CP035974
Coordinates   4765025..4766374 (-) Length   449 a.a.
NCBI ID   WP_131230094.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN102     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4760025..4771374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW31_RS25010 (EXW31_25485) - 4761582..4762520 (-) 939 WP_078173383.1 helix-turn-helix transcriptional regulator -
  EXW31_RS25015 (EXW31_25490) - 4762603..4763046 (+) 444 WP_078179455.1 VOC family protein -
  EXW31_RS25020 (EXW31_25495) hpf 4763133..4763675 (-) 543 WP_002159874.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EXW31_RS25025 (EXW31_25500) cspC 4763997..4764194 (-) 198 WP_033710669.1 cold shock protein CspC -
  EXW31_RS25030 (EXW31_25505) - 4764321..4765025 (-) 705 WP_002112877.1 ComF family protein -
  EXW31_RS25035 (EXW31_25510) comFA 4765025..4766374 (-) 1350 WP_131230094.1 ATP-dependent helicase ComFA Machinery gene
  EXW31_RS25040 (EXW31_25515) - 4766505..4767839 (-) 1335 WP_215598033.1 NlpC/P60 family protein -
  EXW31_RS25045 (EXW31_25520) - 4768072..4768386 (-) 315 WP_002159869.1 winged helix-turn-helix transcriptional regulator -
  EXW31_RS25050 (EXW31_25525) - 4768560..4769402 (-) 843 WP_002112883.1 DegV family protein -
  EXW31_RS25055 (EXW31_25530) - 4769640..4770275 (+) 636 WP_078179458.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50907.16 Da        Isoelectric Point: 9.1493

>NTDB_id=345436 EXW31_RS25035 WP_131230094.1 4765025..4766374(-) (comFA) [Bacillus mycoides strain BPN102]
MLAGRQLLLEELSSELQDKLDHLKENRDVVCVQGVSKKSSKYMCQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGISEISGENYLNPLQWEGVLSAGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQQGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLHYAVNEAM
KEEAARIYLTATPDEKWKRKLRNGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLMRKKIPRALLQWLNMYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVVAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAQEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=345436 EXW31_RS25035 WP_131230094.1 4765025..4766374(-) (comFA) [Bacillus mycoides strain BPN102]
ATGTTAGCTGGTAGACAGTTATTGTTAGAAGAACTTTCTTCAGAGTTACAGGATAAATTGGATCATTTGAAAGAGAATCG
AGACGTCGTTTGTGTACAAGGGGTAAGTAAGAAATCTTCAAAATATATGTGCCAGCGCTGCGGAAATATAGAGCAGAGAC
TATTTGCATCGTTTCTATGCAAAAGGTGCAGTAAAGTATGCACGTATTGCCGGAAGTGCATAACGATGGGCAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTTCTGAAATAAGCGGAGAAAATTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGCTGGTCAGGAGTTAGCGGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAAGCACTACAACAGGGAGAGAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGGTTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCGGATAAGATGTTGCATTATGCAGTAAACGAAGCGATG
AAAGAGGAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAATTGCGGAATGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGTTATCATCGTCATCCATTGCCAGTCCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATGCGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATATGTACTTATCTAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGTCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGTAGCTTTCAGAAAGGGAGAAATCCCTTTATTAGTTACAACGACAATTTTGG
AGCGAGGAGTAACTGTGAAAAATTTACAAGTGGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTCATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTCAAGAACAAGGATTGATAGATTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-409)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.02

98.218

0.501
  comFA Latilactobacillus sakei subsp. sakei 23K

41.266

87.973

0.363

Multiple sequence alignment