Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EXW31_RS20870 Genome accession   NZ_CP035974
Coordinates   3991706..3992452 (-) Length   248 a.a.
NCBI ID   WP_016106175.1    Uniprot ID   R8Q2I7
Organism   Bacillus mycoides strain BPN102     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3986706..3997452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW31_RS20850 (EXW31_21205) dnaG 3987436..3989238 (-) 1803 WP_215572055.1 DNA primase -
  EXW31_RS29615 - 3989320..3989463 (-) 144 WP_227493659.1 hypothetical protein -
  EXW31_RS29620 - 3989439..3989732 (-) 294 WP_234879578.1 DUF188 domain-containing protein -
  EXW31_RS20860 (EXW31_21215) - 3990125..3990937 (-) 813 WP_002160452.1 pyruvate, water dikinase regulatory protein -
  EXW31_RS20865 (EXW31_21220) - 3990967..3991599 (-) 633 WP_087947415.1 helix-turn-helix transcriptional regulator -
  EXW31_RS20870 (EXW31_21225) recO 3991706..3992452 (-) 747 WP_016106175.1 DNA repair protein RecO Machinery gene
  EXW31_RS20875 (EXW31_21230) - 3992486..3992629 (-) 144 WP_000883924.1 YqzL family protein -
  EXW31_RS20880 (EXW31_21235) era 3992806..3993711 (-) 906 WP_002160449.1 GTPase Era -
  EXW31_RS20885 (EXW31_21240) - 3993704..3994102 (-) 399 WP_002111886.1 cytidine deaminase -
  EXW31_RS20890 (EXW31_21245) - 3994202..3994555 (-) 354 WP_078180623.1 diacylglycerol kinase family protein -
  EXW31_RS20895 (EXW31_21250) ybeY 3994552..3995022 (-) 471 WP_002111888.1 rRNA maturation RNase YbeY -
  EXW31_RS20900 (EXW31_21255) - 3995019..3997163 (-) 2145 WP_215569200.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28583.10 Da        Isoelectric Point: 7.7708

>NTDB_id=345426 EXW31_RS20870 WP_016106175.1 3991706..3992452(-) (recO) [Bacillus mycoides strain BPN102]
MFQKVEGIVIRTTDYGETNKIVTIYSREFGKISAMARGAKKPKSRLAAISQLMTHGHFLIQIGSGLGTLQQGEIISSMKE
IREDIFLTAYASFIIELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEALSLIYQTKMLPVLGMHPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFLHFDLHRLGNVSVKDSTKKQMRTVLNTYYDEYCGIYLKTRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=345426 EXW31_RS20870 WP_016106175.1 3991706..3992452(-) (recO) [Bacillus mycoides strain BPN102]
ATGTTTCAAAAAGTTGAGGGCATTGTCATCCGTACAACAGATTACGGAGAAACAAATAAGATTGTTACAATATACTCACG
GGAATTTGGTAAGATAAGTGCAATGGCAAGAGGGGCGAAAAAACCGAAGAGTCGGTTAGCGGCTATTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATTGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCATCTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTATCGAATTAACTGATAAAGCAACAGAAGATAAGAAGCA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGACCCAGAAGCATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCACCCGTACTTTGATACATGTGCTATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCGGAACAAGATCCGTATCGTATTCCGGTGGG
AGAGGCTGTTCATAAATTATTACGTCTCTTTCTTCACTTCGATCTACACCGGCTCGGTAATGTATCGGTGAAGGATAGTA
CAAAAAAACAAATGCGCACAGTATTGAATACATATTATGATGAATATTGTGGGATTTATTTGAAAACAAGGCGTTTCTTG
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8Q2I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment