Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW31_RS19365 Genome accession   NZ_CP035974
Coordinates   3728113..3728547 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN102     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3723113..3733547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW31_RS19350 (EXW31_19675) - 3724277..3724834 (+) 558 WP_088079298.1 PadR family transcriptional regulator -
  EXW31_RS19355 (EXW31_19680) metE 3725355..3727643 (+) 2289 WP_002111534.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW31_RS19360 (EXW31_19685) comJ 3727684..3728073 (-) 390 WP_002111536.1 competence protein ComJ -
  EXW31_RS19365 (EXW31_19690) nucA/comI 3728113..3728547 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  EXW31_RS19370 (EXW31_19695) - 3728784..3729527 (+) 744 WP_002111539.1 DUF3967 domain-containing protein -
  EXW31_RS19375 (EXW31_19700) - 3729587..3730861 (-) 1275 WP_002111540.1 sensor histidine kinase -
  EXW31_RS19380 (EXW31_19705) - 3731170..3731370 (+) 201 WP_002111542.1 hypothetical protein -
  EXW31_RS19385 (EXW31_19710) - 3731591..3732754 (+) 1164 WP_002160658.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=345422 EXW31_RS19365 WP_002014817.1 3728113..3728547(-) (nucA/comI) [Bacillus mycoides strain BPN102]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=345422 EXW31_RS19365 WP_002014817.1 3728113..3728547(-) (nucA/comI) [Bacillus mycoides strain BPN102]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGGCATTCAGAAATGTGCACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAGGGTGGAAA
AGGAGCCCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment