Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW35_RS25715 Genome accession   NZ_CP035965
Coordinates   4978577..4979926 (-) Length   449 a.a.
NCBI ID   WP_033732100.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN09/1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4973577..4984926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW35_RS25680 (EXW35_26205) - 4973713..4974408 (-) 696 WP_002129924.1 hypothetical protein -
  EXW35_RS25685 (EXW35_26210) - 4974395..4975183 (-) 789 WP_078213836.1 hypothetical protein -
  EXW35_RS25690 (EXW35_26215) - 4975215..4975784 (-) 570 WP_098632001.1 hypothetical protein -
  EXW35_RS25695 (EXW35_26220) - 4975903..4976397 (-) 495 WP_002184661.1 hypothetical protein -
  EXW35_RS25700 (EXW35_26225) raiA 4976686..4977228 (-) 543 WP_215550695.1 ribosome-associated translation inhibitor RaiA -
  EXW35_RS25705 (EXW35_26230) cspC 4977549..4977746 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW35_RS25710 (EXW35_26235) - 4977873..4978577 (-) 705 WP_002129929.1 ComF family protein -
  EXW35_RS25715 (EXW35_26240) comFA 4978577..4979926 (-) 1350 WP_033732100.1 ATP-dependent helicase ComFA Machinery gene
  EXW35_RS25720 (EXW35_26245) - 4980057..4981400 (-) 1344 WP_002184658.1 C40 family peptidase -
  EXW35_RS25725 (EXW35_26250) - 4981634..4981948 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW35_RS25730 (EXW35_26255) - 4982122..4982964 (-) 843 WP_002016143.1 DegV family protein -
  EXW35_RS25735 (EXW35_26260) - 4983203..4983838 (+) 636 WP_002034752.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50912.32 Da        Isoelectric Point: 9.4468

>NTDB_id=345389 EXW35_RS25715 WP_033732100.1 4978577..4979926(-) (comFA) [Bacillus mycoides strain BPN09/1]
MLAGRQLLLEELSSDLQDKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGILSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQVDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTIKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=345389 EXW35_RS25715 WP_033732100.1 4978577..4979926(-) (comFA) [Bacillus mycoides strain BPN09/1]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGGATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTACAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCATTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAGTTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGGGGAGTAACTATTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.34

98.218

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

40.955

88.641

0.363

Multiple sequence alignment