Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW35_RS21125 Genome accession   NZ_CP035965
Coordinates   4131696..4132739 (-) Length   347 a.a.
NCBI ID   WP_012261710.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN09/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4126696..4137739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW35_RS21080 (EXW35_21525) - 4127094..4127294 (-) 201 WP_002015009.1 YqzE family protein -
  EXW35_RS21085 (EXW35_21530) - 4127333..4127830 (-) 498 WP_033731265.1 shikimate kinase -
  EXW35_RS21090 (EXW35_21535) - 4127982..4128632 (-) 651 WP_078213471.1 2OG-Fe(II) oxygenase -
  EXW35_RS21095 (EXW35_21540) comGG 4128807..4129178 (-) 372 WP_002167676.1 competence type IV pilus minor pilin ComGG -
  EXW35_RS21100 (EXW35_21545) comGF 4129175..4129636 (-) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  EXW35_RS21105 (EXW35_21550) - 4129620..4129916 (-) 297 WP_002088621.1 hypothetical protein -
  EXW35_RS21110 (EXW35_21555) comGD 4129909..4130364 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  EXW35_RS21115 (EXW35_21560) comGC 4130361..4130660 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW35_RS21120 (EXW35_21565) comGB 4130672..4131709 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  EXW35_RS21125 (EXW35_21570) comGA 4131696..4132739 (-) 1044 WP_012261710.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW35_RS21130 (EXW35_21575) - 4132945..4133640 (+) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  EXW35_RS21135 (EXW35_21580) - 4133922..4134164 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW35_RS21140 (EXW35_21585) - 4134271..4135665 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW35_RS21145 (EXW35_21590) - 4135746..4136138 (-) 393 WP_002190672.1 hypothetical protein -
  EXW35_RS21150 (EXW35_21595) - 4136226..4136441 (-) 216 WP_002067106.1 DUF3912 family protein -
  EXW35_RS21155 (EXW35_21600) - 4136694..4137005 (+) 312 WP_002129099.1 hypothetical protein -
  EXW35_RS21160 (EXW35_21605) - 4137042..4137530 (-) 489 WP_078213472.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39276.79 Da        Isoelectric Point: 9.4642

>NTDB_id=345378 EXW35_RS21125 WP_012261710.1 4131696..4132739(-) (comGA) [Bacillus mycoides strain BPN09/1]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLEASVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345378 EXW35_RS21125 WP_012261710.1 4131696..4132739(-) (comGA) [Bacillus mycoides strain BPN09/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATTATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTGCGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTGTGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAAGCGTCGGTTCGAAAAGGATATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment