Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EXW30_RS00530 Genome accession   NZ_CP035962
Coordinates   97553..98929 (+) Length   458 a.a.
NCBI ID   WP_002159617.1    Uniprot ID   J8FCK3
Organism   Bacillus mycoides strain BPN07/3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92553..103929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW30_RS00510 (EXW30_00515) ctsR 92752..93213 (+) 462 WP_131232662.1 transcriptional regulator CtsR -
  EXW30_RS00515 (EXW30_00520) - 93383..93931 (+) 549 WP_002159618.1 UvrB/UvrC motif-containing protein -
  EXW30_RS00520 (EXW30_00525) - 93936..95000 (+) 1065 WP_002091490.1 protein arginine kinase -
  EXW30_RS00525 (EXW30_00530) clpC 95023..97458 (+) 2436 WP_002063405.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EXW30_RS00530 (EXW30_00535) radA 97553..98929 (+) 1377 WP_002159617.1 DNA repair protein RadA Machinery gene
  EXW30_RS00535 (EXW30_00540) disA 98933..100006 (+) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  EXW30_RS00540 (EXW30_00545) - 100170..101273 (+) 1104 WP_002159616.1 PIN/TRAM domain-containing protein -
  EXW30_RS00545 (EXW30_00550) ispD 101290..101970 (+) 681 WP_016103295.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EXW30_RS00550 (EXW30_00555) ispF 102084..102560 (+) 477 WP_002159614.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49842.55 Da        Isoelectric Point: 7.4250

>NTDB_id=345300 EXW30_RS00530 WP_002159617.1 97553..98929(+) (radA) [Bacillus mycoides strain BPN07/3]
MAKKKTKFTCQECGYQSPKYMGKCPGCGKWNTLVEEMEPVVSSRRLNYVNAIQSEVTKPRRLTEVETNSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=345300 EXW30_RS00530 WP_002159617.1 97553..98929(+) (radA) [Bacillus mycoides strain BPN07/3]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTAAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTATGTCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAATTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGCGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACTGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAGCTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACCAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTCGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TTGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAACCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCAAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAGGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8FCK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment