Detailed information NTG0034524

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PC1_RS14135
• Genome accession: NC_012917
• Coordinates: 3223615..3224460 (-)
• Length: 281 a.a.
• NCBI ID: WP_015841038.1
• Uniprot ID: C6DAP4
• Organism: Pectobacterium carotovorum subsp. carotovorum PC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3218615..3229460

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PC1_RS14115 (PC1_2845)	-	3218810..3219580 (-)	771	WP_015841034.1	ABC transporter ATP-binding protein	-
PC1_RS14120 (PC1_2846)	-	3219582..3220619 (-)	1038	WP_015841035.1	FecCD family ABC transporter permease	-
PC1_RS14125 (PC1_2847)	-	3220616..3221680 (-)	1065	WP_015841036.1	ABC transporter substrate-binding protein	-
PC1_RS14130 (PC1_2848)	-	3222016..3223041 (+)	1026	WP_015841037.1	LLM class flavin-dependent oxidoreductase	-
PC1_RS22385	ypdK	3223398..3223472 (+)	75	WP_099606229.1	membrane protein YpdK	-
PC1_RS14135 (PC1_2849)	pilD	3223615..3224460 (-)	846	WP_015841038.1	prepilin peptidase	Machinery gene
PC1_RS14140 (PC1_2850)	-	3224568..3225314 (-)	747	WP_015841039.1	type II secretion system protein N	-
PC1_RS14145 (PC1_2851)	gspM	3225304..3225798 (-)	495	WP_015841040.1	type II secretion system protein GspM	-
PC1_RS14150 (PC1_2852)	gspL	3225795..3227072 (-)	1278	WP_015841041.1	type II secretion system protein GspL	-
PC1_RS14155 (PC1_2853)	gspK	3227069..3228055 (-)	987	WP_015841042.1	type II secretion system minor pseudopilin GspK	-
PC1_RS14160 (PC1_2854)	gspJ	3228052..3228702 (-)	651	WP_015841043.1	type II secretion system minor pseudopilin GspJ	-
PC1_RS14165 (PC1_2855)	gspI	3228702..3229067 (-)	366	WP_015841044.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31307.46 Da
• Isoelectric Point: 7.1428

>NTDB_id=34524 PC1_RS14135 WP_015841038.1 3223615..3224460(-) (pilD) [Pectobacterium carotovorum subsp. carotovorum PC1]
MDDLREFALVFPAWWYGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELQTGVTDVEHVTSYNLCWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQPVSVQYPLVEVITMLAFLVAGLLWLPGMALCGALILLSFLVVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLRDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGVRKEDTVKPLAFGPWLAIGGVFGVIMNGLYL

Nucleotide

• Length: 846 bp

>NTDB_id=34524 PC1_RS14135 WP_015841038.1 3223615..3224460(-) (pilD) [Pectobacterium carotovorum subsp. carotovorum PC1]
GTGGACGATTTAAGGGAATTCGCACTGGTATTTCCGGCATGGTGGTATGGTGCGCTGGGCGTATTGGGCTTGATTGTTGG
CAGTTTTCTGAACGTCGTGATTTACCGCCTGCCGATTATGTTAGAACGCCGCTGGCGGCAGGACATCGAACTGCAAACGG
GTGTGACCGATGTCGAGCATGTGACGAGTTACAACCTGTGTTGGCCGCCTTCGTCTTGCCCGCACTGTCAGCAGGCGATT
GCGGTGAAAGATAACATCCCGTTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAGCCAGTGTCTGT
GCAGTATCCGCTGGTGGAAGTCATTACGATGCTGGCTTTTCTGGTCGCAGGGTTACTGTGGTTGCCCGGCATGGCGTTAT
GCGGGGCGCTGATTCTGCTGTCCTTTCTGGTGGTTTTGACCGTCATTGATATAAAAACGTTACTGCTGCCAGATGTGCTT
ACGCTTTCGCTCTTGTGGATGGGACTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTACGCGATGCCGTAGTAGGCGC
GATGGCGGGCTATTTGTCCCTGTGGCTACTTTATTGGGTATTTAAATATGCCACAGGCAAAGAAGCGCTAGGGTATGGTG
ATTTTAAGTTACTCGCCGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTCTTGGTGGCGGCGCTGAGC
GGGCTGGTCGTTACTCTCATCTGGCGCGGGGTGCGTAAAGAAGATACGGTTAAACCGTTGGCCTTTGGCCCTTGGCTGGC
GATTGGCGGCGTGTTCGGTGTGATAATGAACGGACTCTATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: C6DAP4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.741	96.085	0.488
pilD	Neisseria gonorrhoeae MS11	49.805	91.459	0.456
pilD	Vibrio campbellii strain DS40M4	46.85	90.391	0.423
pilD	Acinetobacter baumannii D1279779	42.336	97.509	0.413
pilD	Acinetobacter nosocomialis M2	42.336	97.509	0.413