Detailed information NTG0345176

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GFB63_RS01325
• Genome accession: NZ_CP045596
• Coordinates: 275382..276467 (-)
• Length: 361 a.a.
• NCBI ID: WP_011226304.1
• Uniprot ID: Q5M3H6
• Organism: Streptococcus thermophilus strain TK-P3A
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 270382..281467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFB63_RS01295 (GFB63_01350)	-	270746..271561 (-)	816	WP_024010001.1	Cof-type HAD-IIB family hydrolase	-
GFB63_RS01300 (GFB63_01355)	-	271554..272348 (-)	795	WP_011681412.1	HAD-IIB family hydrolase	-
GFB63_RS01305 (GFB63_01360)	-	272533..272910 (+)	378	WP_011681413.1	GntR family transcriptional regulator	-
GFB63_RS01310 (GFB63_01365)	-	272915..273613 (+)	699	WP_193838343.1	ABC transporter ATP-binding protein	-
GFB63_RS01315 (GFB63_01370)	-	273625..274410 (+)	786	WP_011681414.1	hypothetical protein	-
GFB63_RS01320 (GFB63_01375)	-	274460..275389 (-)	930	WP_193838344.1	ATP-binding cassette domain-containing protein	-
GFB63_RS01325 (GFB63_01380)	amiE	275382..276467 (-)	1086	WP_011226304.1	ABC transporter ATP-binding protein	Regulator
GFB63_RS01330 (GFB63_01385)	amiD	276477..277403 (-)	927	WP_084828982.1	oligopeptide ABC transporter permease OppC	Regulator
GFB63_RS01335 (GFB63_01390)	amiC	277403..278896 (-)	1494	WP_024010009.1	ABC transporter permease	Regulator
GFB63_RS01340 (GFB63_01395)	amiA3	278962..280932 (-)	1971	WP_193838345.1	peptide ABC transporter substrate-binding protein	Regulator
GFB63_RS01345 (GFB63_01400)	-	281169..281359 (-)	191	Protein_279	IS3 family transposase	-

Sequence

Protein

• Length: 361 a.a.
• Molecular weight: 39864.62 Da
• Isoelectric Point: 4.7435

>NTDB_id=345176 GFB63_RS01325 WP_011226304.1 275382..276467(-) (amiE) [Streptococcus thermophilus strain TK-P3A]
MTENKNVILSARDIVVEFDVRDRVLTAIRGVSLDLVEGEVLALVGESGSGKSVLTKTFTGMLEENGRVASGSIDYRGKDL
TKFKSHQDWAAIRGAKIATIFQDPMTSLNPIKTIGSQIIEVIVKHQGKTAKEAKKMAIDYMDKVGIPDAEKRFNEYPFQY
SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLKEEYGFSVIFITHDLGVVASIADKVAVMYAGEIIE
YATVEEIFYEPCHPYTWSLLSSLPQLADDNGKLFSIPGTPPSLYTPVVGDAFALRSDYALQIDFEEKAPQFQVSDTHWAK
TWLLHEDAPKVDKPAVIQNLHEKILANMGFAHLGDEEEGNA

Nucleotide

• Length: 1086 bp

>NTDB_id=345176 GFB63_RS01325 WP_011226304.1 275382..276467(-) (amiE) [Streptococcus thermophilus strain TK-P3A]
ATGACAGAAAATAAAAATGTAATATTATCGGCTCGCGATATCGTCGTGGAATTTGACGTTCGTGACCGTGTTTTGACAGC
TATTCGAGGGGTTTCTCTTGATTTAGTTGAAGGTGAAGTTTTAGCCTTGGTTGGTGAGTCTGGTTCAGGAAAATCAGTCT
TGACTAAAACTTTTACAGGGATGCTGGAAGAAAATGGTCGTGTAGCTAGCGGATCTATCGACTACCGTGGCAAAGACTTG
ACTAAATTTAAGAGTCACCAAGATTGGGCAGCTATCCGTGGAGCTAAGATTGCAACTATTTTCCAAGATCCAATGACCAG
TCTTAACCCAATTAAGACTATCGGAAGTCAAATTATTGAAGTTATTGTTAAGCACCAAGGGAAAACAGCCAAGGAAGCTA
AAAAAATGGCAATTGATTACATGGACAAGGTTGGTATTCCAGATGCTGAAAAACGTTTCAATGAATATCCTTTCCAATAC
TCTGGTGGGATGCGTCAACGTATCGTTATTGCCATTGCCTTGGCTTGTCGTCCTGATGTCCTTATCTGTGACGAACCAAC
AACTGCCCTTGATGTGACCATTCAAGCTCAAATTATTGACCTCTTGAAATCACTCAAAGAGGAGTATGGTTTCTCGGTCA
TTTTCATTACCCATGACCTTGGCGTCGTAGCAAGTATCGCGGACAAGGTTGCTGTCATGTATGCTGGCGAAATCATTGAA
TACGCAACTGTTGAGGAAATTTTCTATGAGCCTTGTCATCCATACACGTGGAGCTTGCTTTCAAGTCTGCCACAATTGGC
TGATGATAATGGAAAACTCTTCTCAATTCCAGGGACGCCACCATCACTTTATACACCAGTTGTTGGGGATGCCTTTGCCT
TGCGTTCAGATTATGCTTTGCAGATTGATTTTGAGGAAAAAGCACCACAATTCCAAGTTTCAGATACTCACTGGGCTAAG
ACCTGGCTCTTACATGAGGATGCGCCAAAAGTTGATAAACCTGCGGTTATCCAAAATCTACATGAAAAAATCCTAGCCAA
TATGGGATTTGCACATTTAGGAGATGAGGAGGAAGGCAATGCCTGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-300)

3D structure

• AlphaFold DB: Q5M3H6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus thermophilus LMG 18311	100	100	1
amiE	Streptococcus thermophilus LMD-9	100	100	1
amiE	Streptococcus salivarius strain HSISS4	96.953	100	0.97
oppD	Streptococcus mutans UA159	54.441	96.676	0.526