Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CCO2228_RS05680 Genome accession   NZ_CP035927
Coordinates   1112583..1113527 (+) Length   314 a.a.
NCBI ID   WP_002777230.1    Uniprot ID   A0A3Z8C350
Organism   Campylobacter coli strain RM2228     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1097440..1118335 1112583..1113527 within 0


Gene organization within MGE regions


Location: 1097440..1118335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCO2228_RS05600 (CCO2228_1095) - 1097440..1098369 (-) 930 WP_002777213.1 glycosyltransferase family 2 protein -
  CCO2228_RS05605 (CCO2228_1096) - 1098362..1099438 (-) 1077 WP_002777214.1 glycosyltransferase -
  CCO2228_RS05610 (CCO2228_1097) - 1099435..1101135 (-) 1701 WP_002777215.1 ABC transporter ATP-binding protein -
  CCO2228_RS05615 (CCO2228_1098) galE 1101129..1102115 (-) 987 WP_002777216.1 UDP-glucose 4-epimerase GalE -
  CCO2228_RS05620 (CCO2228_1099) - 1102172..1102963 (-) 792 WP_025443299.1 3'-5' exonuclease -
  CCO2228_RS05625 (CCO2228_1100) waaC 1103028..1104056 (+) 1029 WP_002777218.1 lipopolysaccharide heptosyltransferase I -
  CCO2228_RS05630 (CCO2228_1101) - 1104049..1104936 (+) 888 WP_002777219.1 lipid A biosynthesis lauroyl acyltransferase -
  CCO2228_RS05635 (CCO2228_1102) - 1104933..1106480 (+) 1548 WP_002777220.1 glycosyltransferase -
  CCO2228_RS05640 (CCO2228_1103) - 1106477..1107532 (+) 1056 WP_002777221.1 glycosyltransferase family 4 protein -
  CCO2228_RS05645 (CCO2228_1104) - 1107519..1108730 (-) 1212 WP_002777222.1 glycosyltransferase family 8 protein -
  CCO2228_RS05650 (CCO2228_1105) - 1108718..1109653 (-) 936 WP_002777223.1 glycosyltransferase family 2 protein -
  CCO2228_RS05655 (CCO2228_1106) - 1109734..1110534 (-) 801 WP_002777224.1 class I SAM-dependent methyltransferase -
  CCO2228_RS09050 - 1110662..1110987 (+) 326 Protein_1097 alpha-2,3-sialyltransferase -
  CCO2228_RS09055 - 1110960..1111220 (+) 261 WP_265174491.1 alpha-2,3-sialyltransferase -
  CCO2228_RS09120 - 1111217..1111363 (+) 147 WP_168848228.1 hypothetical protein -
  CCO2228_RS05675 (CCO2228_1107) - 1111703..1112524 (-) 822 WP_002777229.1 glycosyltransferase family 2 protein -
  CCO2228_RS05680 (CCO2228_1108) waaF 1112583..1113527 (+) 945 WP_002777230.1 lipopolysaccharide heptosyltransferase II Regulator
  CCO2228_RS05685 (CCO2228_1109) - 1113520..1114281 (-) 762 WP_002777231.1 glycosyltransferase family 25 protein -
  CCO2228_RS05690 (CCO2228_1110) gmhA 1114281..1114841 (-) 561 WP_002777232.1 D-sedoheptulose 7-phosphate isomerase -
  CCO2228_RS05695 (CCO2228_1111) rfaE1 1114838..1116223 (-) 1386 WP_048813179.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CCO2228_RS05700 (CCO2228_1112) rfaD 1116216..1117169 (-) 954 WP_002777234.1 ADP-glyceromanno-heptose 6-epimerase -
  CCO2228_RS05705 (CCO2228_1113) - 1117166..1117729 (-) 564 WP_002777235.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  CCO2228_RS05710 (CCO2228_1114) - 1117806..1118105 (+) 300 WP_002777238.1 cytochrome c -
  CCO2228_RS05715 (CCO2228_1115) - 1118135..1118335 (-) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36065.60 Da        Isoelectric Point: 10.2686

>NTDB_id=345085 CCO2228_RS05680 WP_002777230.1 1112583..1113527(+) (waaF) [Campylobacter coli strain RM2228]
MKVFIHLPTWLGDAVMASPALYGVYHYFKNAEFILYGSFVSTALFKEFPNAKIIVENKKSRYKQALSLRKKLGKIDLALS
FRSALSSKIILHILKVKQRYFFNKYDFKEEHQVLKYLYFIENSLEIKAHSKDLKLPFKLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAQVALNFSKTHEILIFGAGKAEQELCDEIFHILKEKNVKVKNLCNKTTIKTLCQNIVFCDIFITNDS
GPMHIAAAYKTKTIAIFGPTKFTQTSPWQNQNAKLVHLNLSCMPCMQKTCPLKHHRCMKDLKPEKILEAIQNFT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=345085 CCO2228_RS05680 WP_002777230.1 1112583..1113527(+) (waaF) [Campylobacter coli strain RM2228]
ATGAAAGTTTTTATCCATCTTCCCACTTGGCTAGGCGATGCAGTCATGGCCTCACCTGCTTTATATGGAGTTTATCATTA
TTTTAAAAATGCTGAATTTATCCTTTATGGATCTTTTGTATCTACAGCGCTTTTTAAGGAATTTCCTAATGCTAAAATCA
TTGTAGAAAATAAAAAATCACGCTACAAACAAGCCCTTTCTTTACGCAAAAAACTTGGAAAAATCGATCTAGCCCTTTCT
TTTAGATCAGCCCTTTCCTCTAAGATCATTTTACACATCCTTAAGGTAAAACAAAGATATTTTTTTAACAAATATGATTT
TAAAGAAGAACATCAGGTTTTAAAATATCTTTATTTTATAGAAAATTCATTAGAAATTAAAGCTCATTCTAAGGATTTAA
AACTTCCTTTTAAATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCCTAGGATTAAACCCTGGAGCG
AGTTTTGGAAGTGCTAAAAGATGGGATGCGAGCTATTTTGCACAAGTTGCTTTAAATTTTAGCAAAACTCATGAAATTTT
GATTTTTGGAGCAGGTAAAGCAGAACAAGAACTTTGCGATGAAATTTTTCATATACTAAAAGAAAAAAATGTCAAAGTAA
AAAATCTTTGCAATAAAACCACTATCAAAACCCTTTGTCAAAATATCGTTTTTTGTGATATTTTCATCACAAACGATAGC
GGCCCTATGCACATAGCTGCCGCTTATAAAACAAAAACCATAGCTATTTTTGGGCCTACTAAATTTACCCAAACCTCACC
TTGGCAAAATCAAAATGCAAAATTAGTACATCTAAATTTATCTTGCATGCCTTGCATGCAAAAAACCTGTCCTTTAAAAC
ATCATCGGTGCATGAAAGATTTAAAACCGGAAAAAATTTTAGAAGCAATCCAAAATTTTACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(106-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Z8C350

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

83.067

99.682

0.828

Multiple sequence alignment