Detailed information NTG0344647

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: FPL20_RS01710
• Genome accession: NZ_CP045478
• Coordinates: 333731..334852 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain JD-014
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 328731..339852

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FPL20_RS01690 (FPL20_GE00324)	znuC	329240..329935 (-)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-
FPL20_RS01695 (FPL20_GE00325)	znuA	329991..330950 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
FPL20_RS01700 (FPL20_GE00326)	ycdG	331138..332823 (-)	1686	WP_003234736.1	alpha-glucosidase	-
FPL20_RS01705 (FPL20_GE00327)	ycdF	332848..333624 (-)	777	WP_003246415.1	SDR family oxidoreductase	-
FPL20_RS01710 (FPL20_GE00328)	rapF	333731..334852 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
FPL20_RS01715 (FPL20_GE00329)	cwlK	334975..335478 (+)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
FPL20_RS01720 (FPL20_GE00330)	ycdC	335486..336847 (-)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
FPL20_RS01725 (FPL20_GE00331)	ycdB	337034..338452 (-)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
FPL20_RS01730	-	338626..338757 (+)	132	WP_003246334.1	hypothetical protein	-
FPL20_RS01735 (FPL20_GE00332)	ycdA	338780..339844 (+)	1065	WP_003234750.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=344647 FPL20_RS01710 WP_003246478.1 333731..334852(-) (rapF) [Bacillus subtilis strain JD-014]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=344647 FPL20_RS01710 WP_003246478.1 333731..334852(-) (rapF) [Bacillus subtilis strain JD-014]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405