Detailed information NTG0344428

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: ECET_RS18525
• Genome accession: NZ_CP045457
• Coordinates: 3799163..3800146 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain ET12567
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 3794163..3805146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECET_RS18500 (ECET_19055)	ldrD	3794940..3795047 (-)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
ECET_RS18505 (ECET_19065)	-	3795523..3796464 (+)	942	Protein_3658	amino acid permease	-
ECET_RS18510 (ECET_19070)	-	3796446..3797654 (-)	1209	WP_006250222.1	IS4-like element ISVsa5 family transposase	-
ECET_RS18515 (ECET_19080)	-	3797755..3798132 (+)	378	Protein_3660	transporter	-
ECET_RS18520 (ECET_19085)	dppF	3798162..3799166 (-)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
ECET_RS18525 (ECET_19090)	amiE	3799163..3800146 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
ECET_RS18530 (ECET_19095)	dppC	3800157..3801059 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
ECET_RS18535 (ECET_19100)	dppB	3801069..3802088 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
ECET_RS23610	-	3802252..3802362 (+)	111	Protein_3665	hypothetical protein	-
ECET_RS18540 (ECET_19105)	dppA	3802396..3804003 (-)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=344428 ECET_RS18525 WP_001196486.1 3799163..3800146(-) (amiE) [Escherichia coli strain ET12567]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=344428 ECET_RS18525 WP_001196486.1 3799163..3800146(-) (amiE) [Escherichia coli strain ET12567]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404