Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EWI30_RS02595 Genome accession   NZ_CP035738
Coordinates   547524..548957 (-) Length   477 a.a.
NCBI ID   WP_114978853.1    Uniprot ID   A0A9X5F804
Organism   Enterobacter cloacae strain CZ-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542524..553957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWI30_RS02565 (EWI30_02565) elbB 544056..544709 (+) 654 WP_129997050.1 isoprenoid biosynthesis glyoxalase ElbB -
  EWI30_RS02570 (EWI30_02570) mtgA 544706..545440 (+) 735 WP_114978854.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EWI30_RS02575 (EWI30_02575) npr 545471..545743 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  EWI30_RS02580 (EWI30_02580) rapZ 545740..546594 (-) 855 WP_047347692.1 RNase adapter RapZ -
  EWI30_RS02585 (EWI30_02585) ptsN 546640..547131 (-) 492 WP_063862166.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EWI30_RS02590 (EWI30_02590) hpf 547214..547501 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  EWI30_RS02595 (EWI30_02595) rpoN/rpoN1 547524..548957 (-) 1434 WP_114978853.1 RNA polymerase factor sigma-54 Machinery gene
  EWI30_RS02600 (EWI30_02600) lptB 549005..549730 (-) 726 WP_114978852.1 LPS export ABC transporter ATP-binding protein -
  EWI30_RS02605 (EWI30_02605) lptA 549737..550291 (-) 555 WP_024906352.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  EWI30_RS02610 (EWI30_02610) lptC 550260..550835 (-) 576 WP_114978851.1 LPS export ABC transporter periplasmic protein LptC -
  EWI30_RS02615 (EWI30_02615) kdsC 550832..551398 (-) 567 WP_114978850.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EWI30_RS02620 (EWI30_02620) kdsD 551413..552399 (-) 987 WP_025756696.1 arabinose-5-phosphate isomerase KdsD -
  EWI30_RS02625 (EWI30_02625) - 552413..553390 (-) 978 WP_114978849.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54139.91 Da        Isoelectric Point: 4.3358

>NTDB_id=344150 EWI30_RS02595 WP_114978853.1 547524..548957(-) (rpoN/rpoN1) [Enterobacter cloacae strain CZ-1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARVIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=344150 EWI30_RS02595 WP_114978853.1 547524..548957(-) (rpoN/rpoN1) [Enterobacter cloacae strain CZ-1]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGACAGCAATCCACTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGATACCGCTGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTACAGGATTATCTGATGTGGCAGGTGGAACTGACTCCGTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAAGAGATTGAAGCGGTTCTGAAGCGTATCCAGCGTTTCGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ATGAGGCGCGTGTCATCATCAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAAGAAGCGGTTAATCTGATCCAGTCGCTCGATCCACGCCCCGGACAGTCGATCCAGACCAG
CGAACCTGAGTATGTGATCCCGGATGTACTGGTCAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCACGAAACGACTCTGACAACCAATATATTCGT
AGCAATCTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGAGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTTAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCAATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGACCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment