Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EWH46_RS17085 Genome accession   NZ_CP035708
Coordinates   3747192..3748862 (+) Length   556 a.a.
NCBI ID   WP_149504931.1    Uniprot ID   A0A5C1Q6G1
Organism   Sphaerotilus sulfidivorans strain D-507     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3742192..3753862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWH46_RS17060 (EWH46_17055) argS 3742561..3744246 (+) 1686 WP_149504927.1 arginine--tRNA ligase -
  EWH46_RS17065 (EWH46_17060) - 3744281..3744952 (+) 672 WP_180692801.1 SPOR domain-containing protein -
  EWH46_RS17070 (EWH46_17065) - 3745013..3745681 (+) 669 WP_149504929.1 thiol:disulfide interchange protein DsbA/DsbL -
  EWH46_RS17075 (EWH46_17070) lptA 3745785..3746432 (+) 648 WP_244954360.1 lipopolysaccharide transport periplasmic protein LptA -
  EWH46_RS17080 (EWH46_17075) lptB 3746429..3747184 (+) 756 WP_149504930.1 LPS export ABC transporter ATP-binding protein -
  EWH46_RS17085 (EWH46_17080) rpoN/rpoN1 3747192..3748862 (+) 1671 WP_149504931.1 RNA polymerase factor sigma-54 Machinery gene
  EWH46_RS17090 (EWH46_17085) - 3749074..3749322 (+) 249 WP_149504932.1 hypothetical protein -
  EWH46_RS17095 (EWH46_17090) - 3749285..3749917 (-) 633 WP_220784786.1 DUF4126 domain-containing protein -
  EWH46_RS17100 (EWH46_17095) - 3749995..3752394 (-) 2400 WP_149504934.1 DEAD/DEAH box helicase -
  EWH46_RS17105 (EWH46_17100) - 3752729..3753367 (+) 639 WP_149504935.1 hypothetical protein -

Sequence

Protein


Download         Length: 556 a.a.        Molecular weight: 60493.67 Da        Isoelectric Point: 4.3894

>NTDB_id=344025 EWH46_RS17085 WP_149504931.1 3747192..3748862(+) (rpoN/rpoN1) [Sphaerotilus sulfidivorans strain D-507]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEADEDSGGGPEAVTVEGGTSTAAEREDWSAPGT
DSAGEAPADSGSSGDGESAGVDQLDFEGGDAGSDWDSGQDDYQSLLDVGGTRNGAPDDEDSDWSERSSVAESLPDHLRSQ
LRGMRLAPEDQLLVEALIDSLTEDGYLADPLEEIAERLAVGLELGEALGRDVAPGEVTAVDLDQAELPEAPTPEEAQAAA
ELREELLGRLRCALGWLQNLEPCGVGARDLGECLVLQLREHDATEARRVAILICQGHLELLARRDMKKLVAATGADEDDI
RDAQSLIVTLEPKPGRRFARAEANIVVPDVIVQKAGRGWRVVLNPDVMPRLRINDMYAQALRQHRGQRGADDAGSGATLS
ARLQEARWFVKNILQRFDTIQRVSQAIVERQKGFFTHGEIAMKPLVLREIADELGLHESTISRVTTAKYMATPFGTFELK
YFFGSSLNTEAGGNASSTAVRALIKQFVAAEDQKKPLSDSQLSTMLEEQGIQVARRTVAKYREALKIAPANLRKAI

Nucleotide


Download         Length: 1671 bp        

>NTDB_id=344025 EWH46_RS17085 WP_149504931.1 3747192..3748862(+) (rpoN/rpoN1) [Sphaerotilus sulfidivorans strain D-507]
ATGAAACCTTCCCTCCAGGTCCGCCTGTCGCAGCATCTGGCGCTCACGCCGCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTCTCGACGCTGGAGCTGCATCAGGAAGTCGAGCAGATGCTCGAGCAGAACCCCTTCCTCGAAGCCGACGAGGACAGCG
GCGGCGGCCCGGAGGCGGTCACCGTCGAGGGCGGCACCAGCACGGCCGCCGAGCGCGAGGACTGGAGCGCGCCCGGCACC
GACTCCGCCGGCGAGGCGCCAGCCGACAGCGGCAGCAGCGGCGACGGCGAGAGCGCCGGCGTCGACCAGCTCGACTTCGA
AGGCGGCGACGCCGGCAGCGACTGGGACAGCGGCCAGGACGACTACCAGAGCCTGTTGGACGTCGGCGGCACCCGCAACG
GCGCACCCGACGACGAGGACAGCGACTGGAGCGAGCGCTCCAGCGTCGCCGAGAGCCTGCCCGACCACCTGCGCAGCCAG
CTGCGCGGCATGCGCCTGGCGCCCGAGGACCAGCTGCTGGTCGAGGCGCTGATCGACTCGCTGACCGAGGACGGCTATCT
GGCCGATCCGCTCGAGGAGATCGCCGAGCGCCTGGCCGTGGGCCTGGAGCTGGGCGAGGCGCTCGGCCGCGATGTCGCGC
CGGGCGAGGTCACCGCGGTCGACCTCGACCAGGCCGAGCTGCCCGAGGCCCCGACGCCGGAGGAGGCACAGGCCGCCGCC
GAGCTGCGCGAAGAGCTGCTCGGCCGGCTGCGCTGCGCGCTGGGCTGGCTGCAGAACCTCGAGCCCTGCGGCGTGGGCGC
GCGCGACCTGGGCGAATGCCTGGTGCTGCAGCTGCGCGAGCATGACGCCACCGAGGCGCGCCGGGTCGCGATCCTGATCT
GCCAGGGCCATCTGGAGCTGCTGGCGCGGCGCGACATGAAGAAGCTGGTGGCCGCCACCGGCGCCGACGAGGACGACATC
CGCGACGCGCAGTCGCTGATCGTCACGCTCGAGCCCAAGCCGGGCCGGCGCTTCGCGCGCGCCGAGGCCAACATCGTCGT
GCCCGACGTCATCGTGCAGAAGGCCGGCCGCGGCTGGCGGGTCGTGCTCAATCCGGACGTGATGCCCAGGCTGCGCATCA
ACGACATGTACGCACAGGCGCTGCGCCAGCACCGCGGCCAGCGCGGCGCCGACGACGCCGGCAGCGGCGCGACGCTCTCG
GCGCGGCTCCAGGAGGCGCGCTGGTTCGTCAAGAACATCCTGCAGCGCTTCGACACCATCCAGCGCGTCAGCCAGGCGAT
CGTCGAGCGCCAGAAGGGCTTCTTCACCCACGGCGAGATCGCGATGAAGCCGCTGGTGCTGCGCGAGATCGCCGACGAGC
TGGGCCTGCACGAGTCGACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACCTTCGAGCTGAAG
TACTTCTTCGGCTCGAGCCTGAACACCGAGGCCGGCGGCAACGCCTCCAGCACCGCGGTGCGCGCGCTGATCAAGCAGTT
CGTCGCCGCGGAGGACCAGAAGAAGCCGCTCTCCGACAGCCAGCTCAGCACGATGCTGGAGGAGCAGGGCATCCAGGTCG
CGCGCCGCACGGTCGCGAAATACCGCGAGGCGCTGAAGATCGCGCCAGCCAACCTGCGCAAGGCGATCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(399-554)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-377)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.265

100

0.511

Multiple sequence alignment