Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB54_RS15665 Genome accession   NZ_CP035701
Coordinates   3112365..3113381 (-) Length   338 a.a.
NCBI ID   WP_031778293.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2012AW-0154     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107365..3118381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB54_RS15640 aroK 3108225..3108743 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EPB54_RS15645 pilQ 3108932..3110680 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB54_RS15650 pilP 3110712..3111227 (-) 516 WP_031778292.1 pilus assembly protein PilP Machinery gene
  EPB54_RS15655 pilO 3111217..3111810 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB54_RS15660 pilN 3111803..3112381 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  EPB54_RS15665 pilM 3112365..3113381 (-) 1017 WP_031778293.1 type IV pilus assembly protein PilM Machinery gene
  EPB54_RS15670 - 3113524..3116094 (+) 2571 WP_031778294.1 PBP1A family penicillin-binding protein -
  EPB54_RS15675 oxyR 3116150..3117058 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  EPB54_RS15680 - 3117229..3117957 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37820.32 Da        Isoelectric Point: 6.6637

>NTDB_id=343975 EPB54_RS15665 WP_031778293.1 3112365..3113381(-) (pilM) [Vibrio parahaemolyticus strain 2012AW-0154]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDDQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343975 EPB54_RS15665 WP_031778293.1 3112365..3113381(-) (pilM) [Vibrio parahaemolyticus strain 2012AW-0154]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGATCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment