Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EPB54_RS14750 Genome accession   NZ_CP035701
Coordinates   2933874..2934914 (-) Length   346 a.a.
NCBI ID   WP_005461712.1    Uniprot ID   Q87LJ7
Organism   Vibrio parahaemolyticus strain 2012AW-0154     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2928874..2939914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB54_RS14720 endA 2929008..2929703 (+) 696 WP_025501812.1 deoxyribonuclease I -
  EPB54_RS14725 rsmE 2929828..2930559 (+) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EPB54_RS14730 gshB 2930574..2931524 (+) 951 WP_005425823.1 glutathione synthase -
  EPB54_RS14735 - 2931638..2932201 (+) 564 WP_031778736.1 YqgE/AlgH family protein -
  EPB54_RS14740 ruvX 2932250..2932675 (+) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  EPB54_RS14745 pilU 2932737..2933843 (-) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EPB54_RS14750 pilT 2933874..2934914 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  EPB54_RS14755 - 2934943..2935656 (+) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  EPB54_RS14760 proC 2935776..2936594 (+) 819 WP_083148615.1 pyrroline-5-carboxylate reductase -
  EPB54_RS14765 - 2936647..2937204 (+) 558 WP_005482440.1 YggT family protein -
  EPB54_RS14770 yggU 2937204..2937494 (+) 291 WP_005482461.1 DUF167 family protein YggU -
  EPB54_RS14775 - 2937622..2938053 (+) 432 WP_005461703.1 DUF4426 domain-containing protein -
  EPB54_RS14780 - 2938171..2938773 (+) 603 WP_154119942.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38562.05 Da        Isoelectric Point: 6.5039

>NTDB_id=343968 EPB54_RS14750 WP_005461712.1 2933874..2934914(-) (pilT) [Vibrio parahaemolyticus strain 2012AW-0154]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=343968 EPB54_RS14750 WP_005461712.1 2933874..2934914(-) (pilT) [Vibrio parahaemolyticus strain 2012AW-0154]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACCATCCCAAGTAGCATCCCGACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATCGCGAATGCTGAAAAAGGTTTGGTTTTAGTTACTGGCCCAACGGGTT
CTGGTAAATCGACAACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAGGTTCACCGTGATACCCATAGCTTCCAAAA
CGCGCTACGCAGCGCATTGCGTGAAGACCCGGATGTGATCCTTGTCGGTGAGATGCGTGACAAAGAAACCATCAGCCTAG
CGTTAACGGCGGCGGAAACGGGTCACCTAGTTTTCGGTACGTTGCACACCAGTTCAGCAGCAAAAACCATCGACCGTATC
ATCGATGTGTTCCCAGGTAGTGACAAAGACATGGTGCGTTCCATGTTGTCTGAATCCCTTCGTTCAGTTATCGCGCAAAA
ACTGTTGAAACGTAATGGCGGTGGTCGTATCGCTTGTCACGAAATCATGATGGCAACGCCCGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCGCAGATGTACTCAATCATTCAAACGGGTGCCGCGCATGGAATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCGCAAGGCATGGTTTCGCGCGAAGAAGTCGACAGCAAGATTGGGCTTGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

99.711

0.87
  pilT Vibrio cholerae strain A1552

87.246

99.711

0.87
  pilT Acinetobacter baumannii D1279779

72.727

95.376

0.694
  pilT Acinetobacter baumannii strain A118

72.727

95.376

0.694
  pilT Acinetobacter nosocomialis M2

72.424

95.376

0.691
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

69.733

97.399

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Legionella pneumophila strain Lp02

68.278

95.665

0.653
  pilT Legionella pneumophila strain ERS1305867

68.278

95.665

0.653
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.145

91.908

0.488
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364

Multiple sequence alignment