Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GBG22_RS19190 Genome accession   NZ_CP045206
Coordinates   3913379..3913819 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain DE17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3908379..3918819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBG22_RS19170 (GBG22_19585) aroP 3909379..3910749 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  GBG22_RS19175 (GBG22_19590) ampE 3910792..3911646 (-) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  GBG22_RS19180 (GBG22_19595) ampD 3911643..3912194 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GBG22_RS19185 (GBG22_19600) nadC 3912282..3913175 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GBG22_RS19190 (GBG22_19605) pilA 3913379..3913819 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  GBG22_RS19195 (GBG22_19610) pilB 3913829..3915214 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  GBG22_RS19200 (GBG22_19615) hofC 3915204..3916406 (+) 1203 WP_000157254.1 protein transport protein HofC -
  GBG22_RS19205 (GBG22_19620) guaC 3916441..3917484 (-) 1044 WP_001217338.1 GMP reductase -
  GBG22_RS19210 - 3917640..3917684 (-) 45 WP_120795372.1 protein YacM -
  GBG22_RS19215 (GBG22_19625) coaE 3917709..3918329 (+) 621 WP_001269535.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=343868 GBG22_RS19190 WP_000360914.1 3913379..3913819(+) (pilA) [Escherichia coli strain DE17]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=343868 GBG22_RS19190 WP_000360914.1 3913379..3913819(+) (pilA) [Escherichia coli strain DE17]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363