Detailed information NTG0343736

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GAG93_RS01975
• Genome accession: NZ_CP045190
• Coordinates: 178228..179211 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain NT1F31
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 173228..184211

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GAG93_RS01960 (GAG93_02170)	dppA	174371..175978 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
GAG93_RS01965 (GAG93_02180)	dppB	176286..177305 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GAG93_RS01970 (GAG93_02185)	dppC	177315..178217 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GAG93_RS01975 (GAG93_02190)	amiE	178228..179211 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GAG93_RS01980 (GAG93_02195)	dppF	179208..180212 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GAG93_RS01985 (GAG93_02200)	yhjV	180242..181513 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
GAG93_RS01990 (GAG93_02210)	ldrD	181989..182096 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
GAG93_RS01995 (GAG93_02215)	bcsG	182183..183862 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
GAG93_RS02000 (GAG93_02220)	bcsF	183859..184050 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=343736 GAG93_RS01975 WP_001196486.1 178228..179211(+) (amiE) [Escherichia coli strain NT1F31]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=343736 GAG93_RS01975 WP_001196486.1 178228..179211(+) (amiE) [Escherichia coli strain NT1F31]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404