Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F8M43_RS08495 Genome accession   NZ_CP044498
Coordinates   1623239..1624228 (+) Length   329 a.a.
NCBI ID   WP_003232026.1    Uniprot ID   P51835
Organism   Bacillus subtilis strain ms-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618239..1629228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS08480 acpP 1618436..1618669 (+) 234 WP_003154310.1 acyl carrier protein -
  F8M43_RS08485 rncS 1618809..1619558 (+) 750 WP_003232030.1 ribonuclease III -
  F8M43_RS08490 smc 1619659..1623219 (+) 3561 WP_015715874.1 chromosome segregation protein SMC -
  F8M43_RS08495 pilA 1623239..1624228 (+) 990 WP_003232026.1 signal recognition particle-docking protein FtsY Machinery gene
  F8M43_RS08500 sivC 1624265..1624750 (-) 486 WP_003245834.1 Ig-like domain-containing protein -
  F8M43_RS08505 srpM 1624927..1625259 (+) 333 WP_003238590.1 putative DNA-binding protein -
  F8M43_RS08510 ffh 1625273..1626613 (+) 1341 WP_003232020.1 signal recognition particle protein -
  F8M43_RS08515 rpsP 1626719..1626991 (+) 273 WP_003220815.1 30S ribosomal protein S16 -
  F8M43_RS08520 khpA 1626991..1627236 (+) 246 WP_014476792.1 KH domain-containing protein -
  F8M43_RS08525 ylqD 1627358..1627744 (+) 387 WP_014476793.1 YlqD family protein -
  F8M43_RS08530 rimM 1627749..1628273 (+) 525 WP_003232013.1 ribosome maturation factor RimM -
  F8M43_RS08535 trmD 1628270..1629001 (+) 732 WP_003232011.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36342.52 Da        Isoelectric Point: 5.0164

>NTDB_id=343204 F8M43_RS08495 WP_003232026.1 1623239..1624228(+) (pilA) [Bacillus subtilis strain ms-2]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide


Download         Length: 990 bp        

>NTDB_id=343204 F8M43_RS08495 WP_003232026.1 1623239..1624228(+) (pilA) [Bacillus subtilis strain ms-2]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGTGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCAGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(121-320)

SRP54-type protein, helical bundle domain (SRP54_N)

(34-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P51835

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

43.709

91.793

0.401